Title Amyloid proteins : methods and protocols / edited by Einar M. Sigurdsson, Miguel Calero, María Gasset

Edition 2nd ed Published New York, N.Y. : Humana Press, ©2012

Click on the following:
Springer eBooks

Copies

Description 1 online resource (xv, 548 pages) : illustrations Series Methods in molecular biology ; v. 849 Methods in molecular biology (Clifton, N.J.) ; volume 849 Contents Rapid generation of dityrosine cross-linked A-[beta] oligomers via cu-redox cycling / Adam P. Gunn, Blaine R. Roberts, and Ashley I. Bush -- Application of photochemical cross-linking to the study of oligomerization of amyloidogenic proteins / Dahabada H.J. Lopes [and others] -- Preparation of stable amyloid b-protein oligomers of defined assembly order / Clark Rosensweig [and others] -- Purification and fibrillation of full-length recombinant PrP / Natallia Makarava and Ilia V. Baskakov -- Featuring amyloids with fourier transform infrared and circular dichroism spectroscopies / Miguel Calero and María Gasset -- Quasielastic light scattering study of amyloid b-protein fibrillogenesis / Aleksey Lomakin and David B. Teplow -- Conformations of microtubule-associated protein tau mapped by fluorescence resonance energy transfer / Sadasivam Jeganathan [and others] -- Measuring the kinetics of amyloid fibril elongation using quartz crystal microbalances / Alexander K. Buell, Christopher M. Dobson, and Mark E. Welland -- X-ray fibre diffraction studies of amyloid fibrils / Kyle L. Morris and Louise C. Serpell -- Structural characterization of prefibrillar intermediates and amyloid fibrils by small-angle x-ray scattering / Annette Eva Langkilde and Bente Vestergaard -- Atomic force fluorescence microscopy in the characterization of amyloid fibril assembly and oligomeric intermediates / Valeriy Ostapchenko, Maria Gasset, and Ilia V. Baskakov -- Investigating fibrillar aggregates of tau protein by atomic force microscopy / Susanne Wegmann, Daniel J. Muller, and Eckhard Mandelkow -- Structural studies of amyloids by quenched hydrogen-deuterium exchange by NMR / Marçal Vilar, Lei Wang, and Roland Riek -- Cyclic amplification of prion protein misfolding / Marcelo A. Barria, Dennisse Gonzalez-Romero, and Claudio Soto -- Search for amyloid-binding proteins by affinity chromatography / Miguel Calero, Agueda Rostagno, and Jorge Ghiso -- Establishing the links between A[beta] aggregation and cytotoxicity in vitro using biophysical approaches / Asad Jan and Hilal A. Lashuel -- Preparation of cultured human vascular cells / Ingvar H. Olafsson, Dadi Th. Vilhjalmsson, and Finnbogi R. Thormodsson -- Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy : isolation of smooth muscle and endothelial cells from mouse brain / Sebastien A. Gauthier [and others] -- In vitro assays measuring protection by proteins such as cystatin C of primary cortical neuronal and smooth muscle cells / Sebastien A. Gauthier [and others] -- Study of neurotoxic intracellular calcium signalling triggered by amyloids / Carlos Villalobos [and others] -- Bacterial amyloids / Yizhou Zhou [and others] -- Study of amyloids using yeast / Reed B. Wickner [and others] -- Cell-to-cell transmission of [alpha]-synuclein aggregates / Seung-Jae Lee [and others] -- Subcutaneous adipose tissue biopsy for amyloid protein studies / Per Westermark -- Analysis of s100 oligomers and amyloids / Hugo M. Botelho, Günter Fritz, and Cláudio M. Gomes -- S100A8/A9 amyloidosis in the ageing prostate : relating ex vivo and in vitro studies / Anna L. Gharibyan, Dina Raveh, and Ludmilla A. Morozova-Roche -- Isolation of amyloid by solubilization in water / Dadi Th. Vilhjalmsson, Indiana E. Ingolfsdottir, and Finnbogi R. Thormodsson -- Histological staining of amyloid and pre-amyloid peptides and proteins in mouse tissue / Hameetha B. Rajamohamedsait and Einar M. Sigurdsson -- Pentameric luminescent-conjugated oligothiophene for optical imaging of in vitro-formed amyloid fibrils and protein aggregates in tissue sections / K. Peter R. Nilsson, Mikael Lindgren, and Per Hammarström -- In vivo magnetic resonance imaging of amyloid-[beta] plaques in mice / Youssef Zaim Wadghiri [and others] -- Mouse model for scrapie : inoculation, clinical scoring, and histopathological techniques / Michele A. Di Bari, Romolo Nonno, and Umberto Agrimi -- Biochemical isolation of insoluble tau in transgenic mouse models of tauopathies / Carl Julien, Alexis Bretteville, and Emmanuel Planel -- Tissue processing prior to analysis of alzheimer's disease associated proteins and metabolites, including A[beta] / Stephen D. Schmidt, Ralph A. Nixon, and Paul M. Mathews -- A-[beta] measurement by enzyme-linked immunosorbent assay / Stephen D. Schmidt [and others] -- Cognitive and sensorimotor tasks for assessing functional impairments in mouse models of Alzheimer's Disease and related disorders / Allal Boutajangout [and others] Summary Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word "amyloid" indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second edition of Amyloid Proteins expands upon the previous edition with current, detailed protocols for the preparation of amyloid and its precursors, specific analytical methods for studying these proteins, cell culture models and assays for production of amyloid proteins, and protocols for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories. Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories Analysis eiwitten proteins amyloid Proteins and Enzymes Eiwitten en enzymen Bibliography Includes bibliographical references and index Notes English Print version record Subject Amyloid -- Laboratory manuals Amyloid beta-protein -- Laboratory manuals Cells. Proteins. Anatomy. Cell culture. Biological assay. Amyloid. Methodology. Investigative Techniques Cells Proteins Multiprotein Complexes Publication Formats Analytical, Diagnostic and Therapeutic Techniques and Equipment Macromolecular Substances Anatomy Publication Characteristics Amino Acids, Peptides, and Proteins Chemicals and Drugs Laboratory Manuals Cells, Cultured Biological Assay Amyloid Methods Animal Structures protein. anatomy. methodology. Proteins Methodology Cells Cell culture Biological assay Anatomy Amyloid Amyloid beta-protein Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Sigurdsson, Einar M Calero, Miguel Gasset, María ISBN 9781617795510 1617795518

  Permalink