Title Animal cell biotechnology : methods and protocols / edited by Ralf Pörtner

Edition Third edition Published New York : Humana Press, [2014] ©2014

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Description 1 online resource (xiv, 498 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 1104 Methods in molecular biology (Clifton, N.J.) ; v. 1104. 1064-3745 Contents High-throughput synchronization of mammalian cell cultures by spiral microfluidics / Wong Cheng Lee, Ali Asgar S. Bhagat, and Chwee Teck Lim -- Insect cell line development using Flp-mediated cassette exchange technology / João Vidigal [and others] -- Scalable transient gene expression in adherent mammalian cells using polyethylenimine / Lukas Fliedl and Christian Kaisermayer -- Scalable transient protein expression / Henry C. Chiou [and others] -- DNA profiling and characterization of animal cell lines / Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins -- Tools for high-throughput process and medium optimization / Martin Jordan and Matthieu Stettler -- Designing media for animal cell culture : CHO cells, the industrial standard / Karlheinz Landauer -- Feed optimization in fed-batch culture / Ana Rita Costa [and others] -- Screening and optimization of chemically defined media and feeds with integrated and statistical approaches / Zhihua Xiao [and others] -- Evaluation of solid and porous microcarriers for cell growth and production of recombinant proteins / Maria Elisa Rodrigues [and others] -- Microbioreactors and scale-down models : growth of CHO cells using the pall micro24 microreactor system / Steve R.C. Warr -- Monitoring cell growth, viability, and apoptosis / Michael Butler, Maureen Spearman, and Katrin Braasch -- Metabolic flux estimation in mammalian cell cultures / Chetan T. Goudar [and others] -- Quenching methods for the analysis of intracellular metabolites / Judith Wahrheit and Elmar Heinzle -- NMR methods for metabolomics of mammalian cell culture bioreactors / Nelly Aranibar and Michael D. Reily -- ¹H-NMR protocol for exometabolome analysis of cultured mammalian cells / Tiago M. Duarte [and others] -- Microfluidic perfusion culture / Koji Hattori, Shinji Sugiura, and Toshiyuki Kanamori -- Disposable bioreactors for inoculum production and protein expression / Regine Eibl, Christian Löffelholz, and Dieter Eibl -- Configuration of bioreactors / Dirk E. Martens, Evert J.van den End, and Mathieu Streefland -- Process analytical technology (PAT) in insect and mammalian cell culture processes : dielectric spectroscopy and focused beam reflectance measurement (FBRM) / Damir Druzinec ... [et a.] -- Cell assessment by at-line microscopy / Alexander Babitzky, Patrick Lindner, and Thomas Scheper -- Seed train optimization for cell culture / Björn Frahm -- Vaccine production : upstream processing with adherent or suspension cell lines / Yvonne Genzel [and others] -- Efficient processes for protein expression using recombinant baculovirus particles / Heike Cremer [and others] -- Chromatographic techniques in the downstream processing of proteins in biotechnology / Ruth Freitag -- Virus separation using membranes / Tanja A. Grein, Ronald Michalsky, and Peter Czermak Summary Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes Analysis chemie chemistry celkweek cell culture biotechnologie biotechnology Chemistry (General) Chemie (algemeen) Bibliography Includes bibliographical references and index Notes English Subject Animal cell biotechnology. Cell culture. Cultures (Biology) Technology. Life sciences. Medical laboratory technology. Physical sciences. Culture Techniques Technology Biological Science Disciplines Clinical Laboratory Techniques Technology, Industry, and Agriculture Natural Science Disciplines Technology, Industry, Agriculture Disciplines and Occupations Investigative Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment Cell Culture Techniques Biotechnology Cells, Cultured biological sciences. physical sciences. bioengineering. Technology Physical sciences Medical laboratory technology Life sciences Cultures (Biology) Animal cell biotechnology Cell culture Mechanical Engineering. Engineering & Applied Sciences. Bioengineering. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Pörtner, Ralf, editor. ISBN 9781627037334 1627037330 1627037322 9781627037327

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