Title Renal cancer : methods and protocols / edited by Jack H. Mydlo

Published Totowa, N.J. : Humana Press, ©2001

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Description 1 online resource (xii, 403 pages) : illustrations Series Methods in molecular medicine ; 53 Methods in molecular medicine ; 53. 1543-1894 Contents Renal cell carcinoma / P. Russo -- Pathology of kidney tumors / D.J. Grignon -- Telomerase assay in renal cancer / W.W. Zhang and L.H. Klotz -- Comparative genomic hybridization / J.C. Presti -- Analyzing the FHIT gene by RT-PCR, western blotting, and immunohistochemistry / R. Baffa [and others] -- Detection of apoptosis in renal cell carcinoma / K.J. Pienta and J.F. Williams -- Fluorescence in situ hybridization (FISH) to metaphase and interphase chromosomes / J.A. Macoska -- Polymerase chain reaction detection of DNA sequence deletions / J.A. Macoska -- Matrix metalloproteinases and their inhibitors / A. Kugler, P. Thelen and R.-H. Ringert -- Purification of growth factor mRNA in renal tissues : bFGF-2, FGF-2, TGF [alpha], and EGFR / J.H. Mydlo -- Laser-capture microdissection / G.L. Griewe [and others] -- Expression of c-met and WT-1 / L. Kushner [and others] -- Molecular analysis of the von Hippel-Lindau disease gene / A. Chernoff [and others] -- Experimental models and methods in antibody targeting of renal cell carcinoma / R.L. Vessella [and others] -- Orthotopic model of renal cell carcinoma / D.M. Nanus and D. Engelstein -- Murine animal model / N. Nishisaka [and others] -- Angiogenesis assays / J.H. Mydlo -- Antiangiogenic approaches to renal cell carcinoma / S.C. Campbell, F.K. Reiher and N.D. Smith -- Interferon alpha, GM-CSF-activated T cells, and IL-6 in renal cell carcinoma / B. Patel and A. Belldegrun -- Immunotherapeutic approach to cancer with cutaneous DNA vaccination / W.B. Browne, W.G. Hawkins and J.J. Lewis -- Monoclonal antibodies / E. Oosterwijk -- Treatment strategies in metastatic renal cell carcinoma / P.F. Mulders -- 23assessment of T-cell immune dysfunction in patients with renal cell carcinoma / R.G. Uzzo [and others] -- Transforming growth factor-[Beta]1 as a novel marker of response to therapy for renal cell carcinoma / H.L. Adler -- Static and flow cytometry / R. Madeb, D. Pode and O. Nativ Renal cell carcinoma / P. Russo -- Pathology of kidney tumors / D.J. Grignon -- Telomerase assay in renal cancer / W.W. Zhang and L.H. Klotz -- Comparative genomic hybridization / J.C. Presti -- Analyzing the FHIT gene by RT-PCR, western blotting, and immunohistochemistry / R. Baffa [and others] -- Detection of apoptosis in renal cell carcinoma / K.J. Pienta and J.F. Williams -- Fluorescence in situ hybridization (FISH) to metaphase and interphase chromosomes / J.A. Macoska -- Polymerase chain reaction detection of DNA sequence deletions / J.A. Macoska -- Matrix metalloproteinases and their inhibitors / A. Kugler, P. Thelen and R.-H. Ringert -- Purification of growth factor mRNA in renal tissues : [Beta]FGF-2, FGF-2, TGF [Alpha], and EGFR / J.H. Mydlo -- Laser-capture microdissection / G.L. Griewe [and others] -- Expression of c-met and WT-1 / L. Kushner [and others] -- Molecular analysis of the von Hippel-Lindau disease gene / A. Chernoff [and others] -- Experimental models and methods in antibody targeting of renal cell carcinoma / R.L. Vessella [and others] -- Orthotopic model of renal cell carcinoma / D.M. Nanus and D. Engelstein -- Murine animal model / N. Nishisaka [and others] -- Angiogenesis assays / J.H. Mydlo -- Antiangiogenic approaches to renal cell carcinoma / S.C. Campbell, F.K. Reiher and N.D. Smith -- Interferon alpha, GM-CSF-activated T cells, and IL-6 in renal cell carcinoma / B. Patel and A. Belldegrun -- Immunotherapeutic approach to cancer with cutaneous DNA vaccination / W.B. Browne, W.G. Hawkins and J.J. Lewis -- Monoclonal antibodies / E. Oosterwijk -- Treatment strategies in metastatic renal cell carcinoma / P.F. Mulders -- 23assessment of T-cell immune dysfunction in patients with renal cell carcinoma / R.G. Uzzo [and others] -- Transforming growth factor-[Beta]1 as a novel marker of response to therapy for renal cell carcinoma / H.L. Adler -- Static and flow cytometry / R. Madeb, D. Pode and O. Nativ Summary Because renal cancer can be managed successfully only when localized, there is a great need to develop new treatments for patients with advanced or metastatic disease. In Renal Cancer: Methods and Protocols, Jack H. Mydlo, MD, and a panel of leading clinicians and researchers review every aspect of the latest surgical, medical, and immunological therapies that can be used in the diagnosis and treatment of renal cancer. These broadly experienced investigators also present a practical account of their best basic research methods, including the use of reverse transcriptase PCR combined with genomic hybridization, cadherin, and metalloproteinase expression to reveal important factors in the detection, staging, aggressiveness, and treatment of this disease. Gene therapy, the generation of monoclonal antibodies, and the use of interferon alpha, GM-CSF, and IL-6 are also discussed. In vivo assays are provided for analyzing angiogenesis, anti-angiogenesis, and general renal tumor biology as a prelude to human clinical trials. Comprehensive and pioneering, Renal Cancer: Methods and Protocols offers urologists, medical oncologists, laboratory investigators, and pathologists a practical collection of the major cutting-edge techniques and therapies for renal cancer today, together with a view of the highly promising future of gene therapy Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL In Springer Protocols Subject Kidneys -- Cancer -- Molecular aspects Kidneys -- Diseases. Adenocarcinoma. Urinary organs -- Diseases. Genitourinary organs -- Diseases. Diseases. Renal cell carcinoma. Diagnosis. Analytical, Diagnostic and Therapeutic Techniques and Equipment Kidney Diseases Urologic Neoplasms Adenocarcinoma Urologic Diseases Carcinoma Urogenital Neoplasms Female Urogenital Diseases Male Urogenital Diseases Neoplasms, Glandular and Epithelial Neoplasms by Site Neoplasms Female Urogenital Diseases and Pregnancy Complications Neoplasms by Histologic Type Disease Kidney Neoplasms Carcinoma, Renal Cell Diagnosis disease. illness. diagnosis. MEDICAL -- Oncology. HEALTH & FITNESS -- Diseases -- Cancer. Urinary organs -- Diseases Renal cell carcinoma Kidneys -- Diseases Genitourinary organs -- Diseases Diseases Diagnosis Adenocarcinoma Kidneys -- Cancer -- Molecular aspects Wetenschappelijke technieken. Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Mydlo, Jack H. LC no. 00033590 ISBN 9781592591442 1592591442 0896038289 9780896038288 1280821094 9781280821097 9786610821099 6610821097

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