| Description |
1 online resource (xxxi, 225 pages) : illustrations |
| Series |
Springer Protocols Handbooks, 1949-2448 |
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Springer protocols (Series)
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| Contents |
Screening of actinobacteria for biological activitieso Screening for antimicrobial activityo Cross streak methodo Agar plug methodo Well diffusiono Disc diffusiono Broth dilution method -- MTP Assayo MABAo Screening for antiinfective or anti-inflammatory activityo Screening for anti TB activityo Agar dilutiono LRP assayo Broth dilution methodo Bactec MGIT 960o Screening for anticancer activityo MTT assayo Dual stainingo Neutral red uptake assayo Lactic acid dehydrogenase assayo Tryphan blue uptake assayo DNA fragmentationo Screening for antiviral activityo MTT assayo P24 Assayo Plague assayo Screening for antioxidant activityo DPPH assayo Nitric oxide scavenging activityo Metal chelating assayo Total antioxidant assayo Hydrogen peroxide scavenging assayo Screening for pigment productiono Screening for enzyme inhibitorso Screening for mosquitocidal activityo Screening for enzyme productiono Screening for biosurfactant production -- please mention different methodsHemolytic assay, |
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Bacterial adhesion to hydrocarbons (BATH) assay, drop collapse assay, oilspreading assay, emulsification assay and surface tension measuremento Screening for Quorum Sensing (QSI)o Screening for sideromycins productiono Screening for antibiofilm activity VI. Production of bioproducts from actinobacteriao Fermentation methodso Agar surface fermentationo Solid state fermentationo Submerged fermentationo Production using immobilized cellso Extraction of bioactive metaboliteso Liquid liquid extractiono Solid liquid extractiono Precipitationo Production optimizationo Classical one-factor-at-a-time methodo Plackett-Burman modelo Response Surface Methodology (RSM)o Kinetics of antibiotic production -- add few more kinetic experiments VII. Eliciting cryptic metabolite productiono OSMAC approacho Inducing cryptic metabolite production using elicitorso Epigenetic modifierso Microparticle enhanced cultivation VIII |
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Metabolite profiling of actinobacterial extractso TLCo HPTLCo UV spectral analysiso GC-MS analysiso LC-MS analysis IX. Bioassay guidedisolation and characterization of metabolites from actinobacteriao Bioassay guided isolationo Preparative TLCo Column chromatographyo Preparative HPLCo Characterization of bioactive metabolites from actinobacteriao Physico-chemical#xF0A7; Colour, consistency, solubility#xF0A7; Melting point analysis#xF0A7; Chemical screening#xF0A7; Test for functional groups#xF0A7; CHN analysiso Spectral analysis -- UV, IR and NMRo XRD analysis X |
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Evaluation of actinobacteria for environmental applicationso Heavy metal resistant/accumulationo Pesticide degradation (qualitative & quantitative)o Dye decolourization (qualitative & quantitative)o Plastic degradation(qualitative & quantitative)o Bioleaching of heavy metals from e-wasteo Anticorrosion activityo Antibiofouling activity -- antimicrobial, inhibition of mollusc foot adherence, inhibition ofalgal spore germination, field testingo Screening of Co2 sequestration XI. Evaluation of actinobacteria for agricultural applicationso Pre-experimental screeningo Antagonistic activity against phyto pathogenso PGP properties (IAA production, siderophore production)o Soil fertility enhancement (N2 fixation, P & Zn solubilisation, extracellular enzymeproduction)o Experimental screeningo Non-pathogenic test in plantso Green house experimento Field experiment XII |
| Summary |
This comprehensive laboratory manual describes the various protocols involved in Actinobacterial research. The content is divided into fifteen major sections, each of which is further divided into sub-sections describing the respective aim, principles, materials & methods, protocol, expected results and diagrams. Readers will find essential protocols for e.g. sample collection, isolation, characterization, analysis, profiling and evaluation of Actinobacteria for various applications. Gathering all relevant protocols concerning Actinobacteria, and written by a team of experienced Actinobacterial researchers, it is the first book of its kind |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Actinobacteria.
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Bacteriology.
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Microbial genetics.
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Microbial genomics.
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Biology -- Technique.
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Microbiology.
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microbiology.
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Microbiology (non-medical)
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Biology, life sciences.
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Bacteriology (non-medical)
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Science -- Life Sciences -- Biology -- Microbiology.
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Science -- Life Sciences -- Biology -- General.
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Science -- Biotechnology.
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Science -- Life Sciences -- Bacteriology.
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Actinobacteria
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Bacteriology
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Biology -- Technique
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Microbial genetics
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Microbial genomics
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Microbiology
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Radhakrishnan, Manikkam, author
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Shanmugasundaram, Thangavel, author
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Gopikrishnan, Venugopal, author
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Jerrine, Joseph, author
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| ISBN |
9781071607282 |
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1071607286 |
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