Description |
xxi, 301 pages : illustrations ; 24 cm |
Series |
Practical approach series ; 146 |
|
Practical approach series ; 146
|
Contents |
1. The cell culture laboratory / Caroline B. Wigley --2. Sterilization / Peter L. Roberts -- 3. Culture media / T. Cartwright and G. P. Shah -- 4. Basic cell culture technique and the maintenance of cell lines /James A. McAteer and John Davis -- 5. Primary culture and the establishment of cell lines / Caroline MacDonald -- 6. Specific cell types and their requirements / J. D. Sato, I. Hayashi, J. Hayashi, H. Hoshi, T. Kawamoto, W. L. McKeehan, R. Matsuda, K. Matsuzaki, K. H. G. Mills, T. Okamoto, G. Serrero, D. J. Sussman and M. Kan -- 7. Cloning / John Clarke, Robin Thorpe and John Davis -- 8. The quality control of cell lines and the prevention, detection, and cure of contamination / A. Doyle and B. J. Bolton -- 9. Good laboratory practice in the cell culture laboratory / Stephen J. Froud and Jan Luker --Appendix 1: Culture collections and other resource centres |
Summary |
In the last 20 years cell culture has developed enormously from being used only in specialized areas of research to being the cornerstone of probably the world's fastest-growing industry, biotechnology. The primary aim of this book is to guide the newcomer progressively through all those areas which nowadays are fundamental to the performance of cell culture. The book will also prove useful to the experienced worker. Topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, culture technique, the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination, and good laboratory practice in the cell culture laboratory |
Analysis |
Animals Cells Biotechnology |
Bibliography |
Includes bibliographical references and index |
Subject |
Cell culture -- Laboratory manuals.
|
|
Cell culture -- Laboratory manuals.
|
|
Culture media (Biology) -- Laboratory manuals.
|
|
Cells, Cultured.
|
|
Culture Media.
|
|
Cytological Techniques.
|
|
Sterilization -- methods.
|
Author |
Davis, J. M. (John M.)
|
LC no. |
94018936 |
ISBN |
0199634335 |
|
0199634343 (hbk.) |
|