Description |
xiii, 514 pages : illustrations ; 23 cm |
Series |
Methods in molecular biology ; 58 |
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Methods in molecular biology (Clifton, N.J.) ; 58
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Contents |
DNA Analysis -- The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells -- Restriction Endonuclease Digestion of DNA -- Agarose Gel Electrophoresis -- Capillary Blotting of Agarose Gels -- Random Primed 32P-Labeling of DNA -- Hybridization and Competition Hybridization of Southern Blots -- Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations -- The Preparation of Fluorescein-Labeled Nucleic Acid Probes and Their Detection Using Alkaline Phosphatase-Catalyzed Dioxetane Chemiluminescence -- Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay -- The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhanced Chemiluminescence -- 3?-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection -- The Preparation of Riboprobes -- Native Polyacrylamide Gel Electrophoresis -- Use of Silver Staining to Detect Nucleic Acids -- End-Labeling of DNA Fragments -- RNA Analysis -- Northern Blot Analysis -- RNA Slot Blotting -- The RNase Protection Assay -- Primer Extension Analysis of mRNA -- S1 Mapping Using Single-Stranded DNA Probes -- Nonradioactive In Situ Hybridization for Cells and Tissues -- Gene Cloning -- In Vitro Packaging of DNA -- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors -- The Production of Double-Stranded Complementary DNA for Use in Making Libraries -- Construction of cDNA Libraries -- Screening ? Libraries -- Subcloning Methods -- Subcloning Strategies and Protocols -- Purification of DNA Fragments from Agarose Gels Using Glass Beads -- Transformation of E. coli -- Transformation of Bacteria by Electroporation -- Preparation of Plasmid DNA by Alkaline Lysis -- The Rapid Boiling Method for Small-Scale Preparation of Plasmid DNA -- Plasmid Preparations with Diatomaceous Earth -- PCR Techniques -- Polymerase Chain Reaction -- Inverse Polymerase Chain Reaction -- Polymerase Chain Reaction with Degenerate Oligonucleotide Primers to Clone Gene Family Members -- Cloning PCR Products Using T-Vectors -- Direct Radioactive Labeling of Polymerase Chain Reaction Products -- A Rapid PCR-Based Colony Screening Protocol for Cloned Inserts -- Use of Polymerase Chain Reaction to Screen Phage Libraries -- DNA Sequencing -- Cloning into M13 Bacteriophage Vectors -- Ordered Deletions Using Exonuclease III -- M13 Phage Growth and Single-Stranded DNA Preparation -- Preparation of ssDNA from Phagemid Vectors -- A Rapid Plasmid Purification Method for Dideoxy Sequencing -- DNA Sequencing Using Sequenase Version 2.0 T7 DNA Polymerase -- Pouring Linear and Buffer-Gradient Sequencing Gels -- Electrophoresis of Sequence Reaction Samples -- Direct Sequencing of PCR Products -- Thermal Cycle Dideoxy DNA Sequencing -- Using the Automated DNA Sequencer -- Terminal Labeling of DNA for Maxam and Gilbert Sequencing -- DNA Sequencing by the Chemical Method -- Site-Directed Mutagenesis and Protein Synthesis -- Site-Directed Mutagenesis of Double-Stranded Plasmids, Domain Substitution, and Marker Rescue by Comutagenesis of Restriction Enzyme Sites -- A Protocol for Site-Directed Mutagenesis Employing a Uracil-Containing Phagemid Template -- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System -- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System -- One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin |
Notes |
Spiral bound |
Bibliography |
Includes bibliography |
Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
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English |
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Methods in molecular biology (Clifton, N.J.) no:58 1064-3745 |
Subject |
Cytology.
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Life sciences.
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Nucleic acids -- Laboratory manuals.
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Genetic Techniques.
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Investigative Techniques.
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Nucleic Acids -- methods
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Polymerase Chain Reaction -- methods.
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Sequence Analysis, DNA.
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Author |
Harwood, Adrian J.
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Humana Press.
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LC no. |
95050258 |
ISBN |
0896033317 (hc : alk. paper) |
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089603402X (comb : alk. paper) |
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