| Description |
xxi, 434 pages : illustrations ; 27 cm |
| Contents |
Ch. 1. An Overview of this Manual -- Ch. 2. Protein Structure -- Ch. 3. Tracking the Target Protein -- Ch. 4. Electrophoretic Techniques -- Ch. 5. Getting Started with Protein Purification -- Ch. 6. Membrane and Particulate-Associated Proteins -- Ch. 7. Transfer and Detection of Proteins on Membrane Supports -- Ch. 8. Peptide Mapping and Microsequencing -- Ch. 9. Modified Proteins and Peptides -- Ch. 10. Chromatography -- Ch. 11. Recombinant Protein Techniques -- App. A. Safety Considerations -- App. B. Antibody Preparation -- App. C. Solutions -- App. D. Nucleic Acids -- App. E. Modifications and Motifs -- App. F. Centrifugation -- App. G. Proteases and Proteolytic Enzyme Inhibitors -- App. H. Radioactivity -- App. I. Miscellaneous -- App. J. List of Suppliers, Vendors, Manufacturers |
| Summary |
This comprehensive, practical and user-friendly manual is designed to be a practical progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. It will also appeal to experienced researchers and graduate students as a benchtop handbook which, without sacrificing scientific concepts, maintains the spirit of experimentation |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Proteins -- Analysis -- Laboratory manuals.
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Proteins -- Purification -- Laboratory manuals.
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Proteins -- analysis.
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| Genre/Form |
Laboratory Manual.
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Laboratory manuals.
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| LC no. |
94023004 |
| ISBN |
081763665X (s : alk. paper) |
|
0817637176 (alk. paper) |
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376433665X (s : alk. paper) |
|
3764337176 (alk. paper) |
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