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Title RNA isolation and characterization protocols / edited by Ralph Rapley and David L. Manning
Published Totowa, N.J. : Humana Press, [1998]
©1998

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Location Call no. Vol. Availability
 W'PONDS  572.88 Rap/Ria  AVAILABLE
Description xiii, 264 pages ; 24 cm
Series Methods in molecular biology ; v. 86
Methods in molecular biology (Clifton, N.J.) ; v. 86
Contents Machine derived contents note: Introduction to Isolating RNA -- Donald E. Macfarlane and Christopher E. Dahle. -- Large and Small Scale RNA Preparations from Eukaryotic Cells -- Wolfgang Uckert, Wolfgang Walther, and Ulrike Stein. -- An Improved Rapid Method of Isolating RNA from Cultured Cells -- David B. Batt, Gordon G. Carmichael, and Zhong Liu. -- Isolating RNA with the Cationic Surfactant, Catrimox-14 -- Christopher E. Dahle and Donald E. Macfarlane. -- RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues -- Giorgio Stanta, Serena Bonin, and Rosella Perin. -- Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides -- Shu-Hua Cheng and Jeffrey R. Seemann. -- Isolation of Plant Mitochondrial RNA from Green Leaves -- Fei Ye, Wolfgang O. Abel, and Ralf Reski. -- Extraction from Fresh and Frozen Blood -- Bimal D. M. Theophilus. -- Isolation of Total RNA from Bacteria -- John Heptinstall. -- Isolation of Total RNA from Tissues or Cell Lines: Visualization in Gel -- Tapas Mukhopadhyay and Jack A. Roth. -- Isolation of Messenger RNA -- Sian Bryant and David L. Manning -- Formaldehyde Gel Electrophoresis of Total RNA -- Sian Bryant and David L. Manning. -- UV Spectrophotometric Analysis of Ribonucleic Acids -- Ralph Rapley and John Heptinstall. -- Preparation of RNA Dot-Blots -- Rachel Hodge. -- Nonradioactive Northern Blotting -- Rainer Lw̲. -- The Use of RNA Probes for the Analysis of Gene Expression -- Dominique Belin. -- Analysis of RNA by Northern Blotting Using Riboprobes -- Rai Ajit K. Srivastava. -- RNA Quantitative Analysis form Fixed and Paraffin-Embedded Tissues -- Giorgio Stanta, Serena Bonin, and Ren ̌Utrera. -- Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing -- Anu Suomalainen and Ann-Christine Syvñen. -- Preparation of Tissue Sections and Slides for mRNA Hybridization -- Giorgio Terenghi. -- Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes -- Giorgio Terenghi . -- One-Tube RT-PCR with Sequence-Specific Primers -- Ulrich Pfeffer -- Identification of Differentially Expressed Genes by Nonradioactive Differential Display of Messenger RNA -- Thomas C. G. Bosch and Jan U. Lohmann. -- Characterization of RNA Using Continuous RT-PCR Coupled with ELISA -- Franȯis Mallet, Guy Oriol, and Bernard Mandrand. -- Gene Expression Analysis by CD-RT-PCR -- Eric de Kant. -- Primer Extension Analysis of mRNA -- Maggie Walmsley, Mark Leonard, and Roger Patient. -- S1 Mapping Using Single-Stranded DNA Probes -- Stp̌hane Viville and Roberto Mantovani. -- Measurements of Rate of Transcription in Isolated Nuclei by Nuclear "Run-Off" Assay -- Rai Ajit Srivastava and Gustav Schonfeld. -- Transcription In Vitro Using Bacteriophage RNA Polymerases -- Elaine T. Schenborn. -- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System -- Louise Olliver and Charles D. Boyd. -- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System -- Louse Olliver, Ann Grobler-Rabie, and Charles D. Boyd. -- The Xenopus Egg Extract Translation System -- Glenn M. Matthews and Alan Colman. -- Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization -- Lesley-Ann Martin and Peter P. C. Mertens. -- Index
Bibliography Includes bibliographical references and index
Notes Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press
Methods in molecular biology (Clifton, N.J.) no:86 1064-3745
Subject RNA -- Analysis -- Laboratory manuals.
RNA -- Purification -- Laboratory manuals.
Author Manning, David L.
Rapley, Ralph.
Humana Press.
LC no. 98012453
ISBN 0896033937 (comb : alk. paper)
0896034941 (hard : alk. paper)