| Description |
xix, 481 pages : illustrations ; 24 cm |
| Series |
Methods in molecular biology ; v. 118 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 118
|
| Contents |
1. Labeling and Purification of RNA Synthesized by In Vitro Transcription / Paul A. Clarke -- 2. Joining RNA Molecules with T4 DNA Ligase / Melissa J. Moore -- 3. RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs / Michelle M. Hanna, Lori Bentsen and Michael Lucido / [et al.] -- 4. The Methylene Blue Mediated Photocrosslinking Method for Detection of Proteins that Interact with Double-Stranded RNA / Zhi-Ren Liu and Christopher W. J. Smith -- 5. Probing RNA-Protein Interactions by Psoralen Photocrosslinking / Zhuying Wang and Tariq M. Rana -- 6. Identification and Sequence Analysis of RNA-Protein Contact Sites by N-Terminal Sequencing and MALDI-MS / Bernd Thiede, Henning Urlaub and Helga Neubauer / [et al.] -- 7. RNA Footprinting and Modification Interference Analysis / Paul A. Clarke -- 8. Oligonucleotide-Targeted RNase H Protection Analysis of RNA-Protein Complexes / Arthur Gunzl and Albrecht Bindereif -- 9. Nitrocellulose Filter Binding for Determination of Dissociation Constants / Kathleen B. Hall and James K. Kranz -- 10. Measuring Equilibrium and Kinetic Constants Using Gel Retardation Assays / David R. Setzer -- 11. PACE Analysis of RNA-Peptide Interactions / Christopher D. Cilley and James R. Williamson -- 12. Detection of Nucleic Acid Interactions Using Surface Plasmon Resonance / Robert J. Crouch, Makoto Wakasa and Mitsuru Haruki -- 13. An Escherichia coli-Based Genetic Strategy for Characterizing RNA Binding Proteins / Chaitanya Jain -- 14. Screening RNA-Binding Libraries Using a Bacterial Transcription Antitermination Assay / Kazuo Harada and Alan D. Frankel -- 15. In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display / Ite A. Laird-Offringa -- 16. In Vitro Selection of Aptamers from RNA Libraries / Daniel J. Kenan and Jack D. Keene -- 17. Identification of Specific Protein-RNA Target Sites Using Libraries of Natural Sequences / Lucy G. Andrews and Jack D. Keene -- 18. Northwestern Screening of Expression Libraries / Paramjeet S. Bagga and Jeffrey Wilusz -- 19. Screening Expression Libraries with Solution-Based Assay / Philippa J. Webster and Paul M. Macdonald -- 20. An Immunoprecipitation-RNA:rPCR Method for the In Vivo Isolation of Ribonucleoprotein Complexes / Edward Chu, John C. Schmitz and Jingfang Ju / [et al.] -- 21. Purification and Depletion of RNP Particles by Antisense Affinity Chromatography / Benjamin J. Blencowe and Angus I. Lamond -- 22. Purification of U Small Nuclear Ribonucleoprotein Particles / Berthold Kastner and Reinhard Luhrmann -- 23. Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes / Maurice S. Swanson and Gideon Dreyfuss -- 24. Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing / Akila Mayeda and Adrian R. Krainer -- 25. Mammalian In Vitro Splicing Assays / Akila Mayeda and Adrian R. Krainer -- 26. Yeast Pre-mRNA Splicing Extracts / Stephanie W. Ruby -- 27. Prespliceosome and Spliceosome Isolation and Analysis / Laura A. Lindsey and Mariano A. Garcia-Blanco -- 28. A Yeast Spliceosome Assay / Stephanie W. Ruby -- 29. Defining Pre-mRNA cis Elements that Regulate Cell-Specific Splicing / Thomas A. Cooper -- 30. In Vivo SELEX in Vertebrate Cells / Thomas A. Cooper -- 31. Purification of SR Protein Splicing Factors / Alan M. Zahler -- 32. Processing mRNA 3' Ends in Vitro / Michael J. Imperiale -- 33. Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay / Fernando J. Salles and Sidney Strickland -- 34. In Vitro Translation Extracts from Tissue Culture Cells / Kazuko Shiroki and Akio Nomoto -- 35. Messenger RNA Turnover in Cell-Free Extracts from Higher Eukaryotes / Jeff Ross |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
| Subject |
RNA-protein interactions -- Laboratory manuals.
|
| Author |
Haynes, Susan R.
|
|
Humana Press.
|
| LC no. |
98055324 |
| ISBN |
0896035689 acid-free paper |
|
