| Description |
xvii, 150 pages : illustrations (some color) ; 25 cm |
| Series |
Springer Nature Book Archives Millennium (2000-2004)
|
| Contents |
Basic Methods. 1. Basic Flow Cytometry Instrumentation / Charles L. Goolsby and Cathy James. 2. Fixation for In Situ Molecular Analysis / Bruce K. Patterson and Daniel K. Jiyamapa. 3. A Method for Reduction of Green Wavelength Autofluorescence Emission in Cellular Preparations / Victoria L. Mosiman. 4. Gene Quantification: Choosing the Target / Bruce K. Patterson. 5. Detection and Quantification of Cytokine-Producing Cells by Immunostaining / Ulf Andersson, Mark J. Litton and Tom E. Fehniger / [et al.]. 6. In Situ Hybridization Using the bDNA Technology / Vincent P. Antao, Audrey N. Player and Janice A. Kolberg -- Specific Applications. 7. Kaposi's Sarcoma - Associated Human Herpes Virus Eight: Localization of Viral Gene Expression and Viral Latency / Scott J. Brodie, John N. Krieger and Catherine Diamond. 8. Pathogenetic Mechanisms of Animal Orbiviruses That Cause Disease at Low Copy Number / Scott J. Brodie, Patricia M. O'Hearn and Kurt Diem / [et al.] |
|
9. Localization of Hepatitis C Virus Using Reverse Transcriptase Fluorescence in Situ 5' Nuclease Assay / Bruce K. Patterson. 10. Simultaneous Quantification and Localization of HIV gag-pol Expression Using Ultrasensitive Fluorescence In Situ Hybridization / Bruce K. Patterson. 11. Quantification and Localization of Gene Expression: Future Directions / Bruce K. Patterson |
| Summary |
"Techniques in Quantification and Localization of Gene Expression describes in detail the methods used to quantify and localize gene expression. As an organism's DNA can be considered to be its genetic potential, its RNA and protein expression are the functional result of its genetic program. Localizing gene expression is therefore an important tool in basic cell and molecular biology research and is particularly important in helping to understand the molecular basis of disease." "Sensitive detection of gene expression is hindered by poor recovery or preservation of target sequences. This book emphasizes methods that are optimized to ensure full recovery and preservation of gene expression targets from specimen acquisition through to detection and final analysis." "Researchers and students in cellular biology will find this book a very useful guide to gene localizing techniques."--BOOK JACKET |
| Notes |
Title from content provider |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Gene expression -- Research -- Methodology.
|
| Author |
Patterson, Bruce K.
|
| LC no. |
99027046 |
| ISBN |
0817640347 alkaline paper |
|
