| Description |
xi, 221 pages : illustrations ; 23 cm |
| Series |
Methods in molecular biology ; v. 65 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 65
|
| Contents |
Machine derived contents note: Preparation and Analysis of DNA Sequencing Gels -- Purification of PCR Products from Agarose Gels for Direct Sequencing -- Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing -- Direct Sequencing of Double-Stranded PCR Products with the Sequencase Kit and [a-35S] dATP -- Direct Sequencing by Thermal Asymmetric PCR -- Rapid Sequencing of cDNA Clones: Direct Sequencing Using Sequential Linear/Asymmetric PCR -- Direct Sequencing of PCR Products Using Chemiluminescent Detection -- Direct DNA Sequencing of PCR Products Using Magnetic Beads -- Affinity Capture and Solid-Phase Sequencing Biotinylated PCR Products -- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing. -- Nonradioactive PCR Sequencing Using Digoxygenin -- Silver SequencingTM: Nonradioactive Cycle Sequencing of dsDNA -- Direct Sequencing of PCR Products with DNA-Binding Proteins -- PCR Sequencing with the Aid of Detergents -- Direct Sequencing with Highly Degenerate and Inosine-Containing Primers -- Determination of Unknown Genomic Sequences Without Cloning -- DNA Sequencing by the Chemical Method -- Direct PCR Sequencing with Denaturants (Formamide) -- Efficient PCR Production of Single-Stranded DNA Sequencing Templates -- Preparation and Direct Automated Cycle Sequencing of PCR Products -- Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA -- Cloning PCR Products for Sequencing in M13 Vectors -- Sequencing PCR Products Cloned into M13 Vectors -- Genome Amplification with Transcript Sequencing (GAWTS) -- DNA Rescue by Vectorette Method -- Sequencing (dAidT) of Cloned Mixed PCR Products from Microbial Populations -- Index |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
| Subject |
Polymerase chain reaction -- Laboratory manuals.
|
| Author |
Rapley, Ralph.
|
|
Humana Press.
|
| LC no. |
96027724 |
| ISBN |
0896033449 (acid-free paper) |
|
