| Description |
214 pages : illustrations ; 24 cm |
| Series |
Current innovations in molecular biology series ; v. 4 |
|
Current innovations in molecular biology series ; v. 4
|
| Contents |
Ch. 1. Adjustment of Parameters in the Yeast Two-Hybrid System: Criteria for Detecting Physiologically Significant Protein-Protein Interactions / Erica A. Golemis, Ilya Serebriiskii and Susan F. Law -- Ch. 2. pJuFo: A Phage Surface Display System for Cloning Genes Based on Protein-ligand Interaction / Reto Crameri -- Ch. 3. Restriction Endonuclease-based Gene Expression Fingerprinting / N. B. Ivanova and A. V. Belyavsky -- Ch. 4. PNA Used for Specific Capture of Nucleic Acids / Martin B. Borre, Michael Naesby and Henrik Orum -- Ch. 5. A PCR-based Method for Isolation of Genomic DNA Flanking a Known DNA Sequence / Catherine A. Boulter and Dipa Natarajan -- Ch. 6. Affinity Selection of Eukaryotic mRNAs and Full-length cDNAs by a CAP Retention Procedure (CAPture) / Jerry Pelletier -- Ch. 7. RNA Ligase-mediated Race: An Effective Method for the Cloning of Full-length cDNA Ends / Brian C. Schaefer |
|
Ch. 8. Combinatorial Scanning Site-directed Mutagenesis / Jean Chatellier and Thierry Vernet -- Ch. 9. A Rapid Method for Creating Gene Mutations and Fusions Using PCR-ligation-PCR Mutagenesis / Stuart Alvaro Ali and Alexander Steinkasserer -- Ch. 10. Double Replacement Gene Targeting in Embryonic Stem Cells for the Introduction of Subtle Alterations into Endogenous Mouse Genes / David W. Melton -- Ch. 11. Universal TA Cloning / Ming-Yi Zhou and Celso E. Gomez-Sanchez -- Ch. 12. Long PCR: Rapid Restriction Mapping and Selective Suppression by Restriction Digestion / Chengtao Her and Richard M. Weinshilboum -- Ch. 13. High-resolution Silver Staining-based PCR-single Strand Conformation Polymorphism (SSCP) Analysis / Langxing Pan and Huaizheng Peng |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Molecular cloning -- Laboratory manuals.
|
|
Molecular cloning.
|
| Author |
Schaefer, Brian C.
|
| LC no. |
97166558 |
| ISBN |
1898486069 |
|
1898486115 |
|
