| Description |
xi, 193 pages ; 22 cm |
| Series |
Essential techniques series |
|
Essential techniques series.
|
| Contents |
I. Introduction -- II. Preparation of DNA -- Protocols. 1. Preparation of cell pellet and cell lysis. 2. Phenol extraction of DNA. 3. Ethanol precipitation of DNA. 4. Removal of RNA by ribonuclease treatment. 5. Final purification steps. 6. Assessment of DNA concentration and DNA quality -- III. Preparation of RNA -- Protocols. 7. Preparation of cell pellet. 8. Preparation of cytoplasmic RNA. 9. Preparation of whole cell RNA by ultracentrifugation method. 10. Preparation of whole cell RNA by Chomczynski-Sacchi method. 11. Assessment of RNA concentration and RNA quality. 12. Selection of poly(A)[superscript +] RNA -- IV. Preparation of plasmid DNA -- Protocols. 13. Transformation of bacteria with plasmid. 14. Growth of bacteria and amplification of plasmid. 15. Small-scale preparation of plasmid DNA. 16. Large-scale preparation of plasmid DNA. 17. Isolation of cloned gene sequences -- V. DNA analysis by restriction enzyme digestion and Southern blotting -- Protocols |
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18. Restriction endonuclease digestion. 19. Concentration of DNA by ethanol precipitation. 20. Agarose gel electrophoresis. 21. Southern blotting. 22. Radiolabeling of DNA probe. 23. Hybridization of blot. 24. Washing of blot. 25. 5'-end radiolabeling of DNA molecular weight markers -- VI. DNA analysis by PCR -- Protocols. 26. PCR reaction. 27. Analysis of PCR products by agarose gel electrophoresis -- VII. RNA analysis by Northern blotting -- Protocols. 28. Formaldehyde-agarose gel electrophoresis. 29. Northern blotting and hybridization to radiolabeled DNA probe -- VIII. RNA analysis by RNase protection -- Protocols. 30. Preparation of riboprobe. 31. RNase protection assay. 32. Analysis of products by polyacrylamide gel electrophoresis -- IX. RNA analysis by RT-PCR and differential display -- Protocols. 33. RT-PCR reaction and analysis of products. 34. Differential display -- X. Analysis of protein-DNA interactions by gel shift assay -- Protocols |
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35. Preparation of protein extract. 36. Gel shift assay -- XI. Gene transfection -- Protocols. 37. Transient transfection. 38. Stable transfection -- App. A. Recipes for routine solutions -- App. B. Sources of reagents and equipment |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Molecular biology -- Laboratory manuals.
|
| LC no. |
98027199 |
| ISBN |
0471977055 (alk. paper) |
|
