| Description |
xvii, 272 pages : illustrations ; 24 cm |
| Series |
Methods in enzymology, 0076-6879 ; v. 541 |
|
Methods in enzymology ; v. 541
|
| Contents |
Section 1: Protein protocols/protein precipiation: TCA precipitation -- Section II: Protein protocols/protein pull-down methods: Coimmunoprecipitation of proteins from yeast -- Coupling antibody to cyanogen bromide-activated sepharose -- Analysis of protein-protein interactions by coimmunoprecipitation --Section III: Protein protocols/protein purification: Use and application of hydrophobic interaction chromatography for protein purification -- Hydroxyapatite chromatography: purification strategies for recombinant proteins -- Salting out of proteins using ammonium sulfate precipitation -- Using ion exchange chromatography to purify a recombinantly expressed protein -- Gel filtration chromatography (size exclusion chromatography) of proteins -- Section IV: Protein protocols/purification of membrane proteins: Expression and purification of membrane proteins -- Explantory chapter: choosing the right detergent -- Section V: Protein protocols/SDS PAGE: One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE) -- Coomassie blue staining -- Silver staining of SDS-polyacrylamide gel -- Section VI: Protein protocols/Standard in vitro assays for protein-nucleic acid interactions: Standard in vitro assays for protein-nucleic acid interactions-gel shift assays for RNA and DNA binding -- Protein filter binding -- Section VII: Protein protocolstroubleshooting protein expression: Explanatory chapter: troubleshooting recombinant protein expression: general -- Explanatory chapter: troubleshooting protein expression: what to do when the protein is not soluble -- Section VIII: Protein protocols/Western blotting: Western blotting using chemiluminescent substrates |
| Bibliography |
Includes bibliographical references and indexes |
| Subject |
Proteins -- Analysis.
|
|
Proteins -- Purification.
|
| Author |
Lorsch, Jon, editor
|
| ISBN |
0124201199 (hardback) |
|
9780124201194 (hardback) |
|
