| Description |
xii, 165 p. : ill. ; 25 cm. + 1 CD-ROM (4 3/4 in.) |
| Contents |
Introduction to LC/MS -- Why LC/MS? -- Molecular Weights and Structure Studies -- LC/MS Systems -- System Costs -- Competitive Systems -- The HPLC System -- HPLC System Components -- Gradient versus Isocratic Systems -- Micro HPLC Systems -- HPLC Tubing and Fittings -- The HPLC Column and Separation Modes -- Column Construction -- Column Packing Materials -- Normal-Phase Columns -- Other Bonded-Phase Silica Columns -- Optimizing Reverse-Phase Column Use -- Silica Ion-Exchange Columns -- Silica Size-Separation Columns -- Zirconium Bonded-Phase Columns -- Polymer Reverse-Phase Columns -- HPLC and Column Maintenance -- HPLC Maintenance -- Column Maintenance -- Sample Preparation and Separations Development -- Mobile-Phase Preparation -- Mobile-Phase pH Control Using Buffers -- Sample Preparation -- Cartridge Column Cleanup -- On-Column Sample Concentration -- Isocratic and Gradient Methods Development -- Automated Methods Development -- LC/MS Interfaces -- Solvent Removal and Ionization -- Atmospheric-Pressure Interfaces -- Electrospray Interface -- Ion Spray Interface -- Secondary Detectors -- LC/MS Overview -- HPLC and the Ionization Source -- Vacuum Pumps -- Analyzer and Ion Detector Designs -- Data and Control Systems -- Peak Detection, ID, and Quantitation -- Mass Analyzers -- Quadrupole Analyzer -- Ion Trap Analyzer -- Linear Ion Trap Analyzer -- Time-of-Flight Analyzer -- Fourier Transform Analyzer Design -- Magnetic Sector Analyzers -- Mass Spectrometer Maintenance -- High-Vacuum Operation |
|
MS Hardware Maintenance -- System Electrical Grounding -- Application Areas for LC/MS -- Compound Discovery -- Identification of Complex Biological Compounds -- Analysis of Trace Impurities and Metabolites -- Arson Residue Investigation -- Industrial Water and Pesticide Analysis -- Toxicology and Drugs of Abuse -- Clinical Therapeutic Drug Screening -- Pesticide Manufacturing -- Trace Analysis and LC/MS/MS -- LC/MS/MS Triple-Quadrupole System -- MS/MS Operating Modes -- Ion Trap MS/MS Operation -- Hybrid LC/MS/MS Systems -- Drug Discovery and Benchtop LC/MS -- Activity Screening -- Standardized LC/MS Screening -- Molecular Fragmentation for Structural Determination -- Process Monitoring -- Proteomics: LC/MALDI/TOF and MS/MS Libraries -- Protein Molecular-Weight Determination by LC/MS -- De Novo Protein Purification -- Protein Analysis by Two-Dimensional GEP and LC/TOFMS -- LC/MS/MS Identification of Peptide Structures -- Tracer Labeling for Peptide ID -- Posttranslational Modified Protein -- Transient Peptides and Accumulation Proteins -- The Future of LC/MS -- Instrumentation Improvements -- Affordable Benchtop LC/LITMS -- User-Customized Data Libraries -- Nucleomics and Restriction Fragment Analysis -- LC/MS Frequently Asked Questions -- Solvents and Volatile Buffers for LC/MS -- Guide to Structure Interpretation -- LC/MS Selective Reading List |
| Bibliography |
Includes bibliographical references (p. 155-156) and index |
| Subject |
Liquid chromatography -- Handbooks, manuals, etc
|
|
High performance liquid chromatography -- Handbooks, manuals, etc
|
|
Mass spectrometry -- Handbooks, manuals, etc
|
|
High performance liquid chromatography.
|
|
Liquid chromatography.
|
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Mass spectrometry.
|
| Genre/Form |
Handbooks and manuals.
|
| LC no. |
2004063820 |
| ISBN |
0471655317 |
|
9780471655312 |
|
