| Description |
1 online resource (x, 273 pages) : illustrations |
| Series |
Springer protocols |
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Methods in molecular biology, 1940-6029 ; 676 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 676. 1064-3745
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| Contents |
Part I. Reviews ; MicroRNA biogenesis and cancer / Julia Winter and Sven Diederichs -- Computational identification of miRNAs involved in cancer / Anastasis Oulas, Nestoras Karathanasis, and Panayiota Poirazi -- The principles of miRNA-masking antisense oligonucleotides technology / Zhiguo Wang -- The concept of multiple-target anti-miRNA antisense oligonucleotide technology / Zhiguo Wang -- Modulation of microRNAs for potential cancer therapeutics / Wei Wu -- Part II. Protocols ; Detection of microRNAs in cultured cells and paraffin-embedded tissue specimens by in situ hybridization / Ashim Gupta and Yin-Yuan Mo -- MicroRNA northern blotting, precursor cloning, and Ago2-improved RNA interference / Julia Winter and Sven Diederichs -- MiRNA profiling on high-throughput OpenArray"!system / Elena V. Grigorenko, Elen Ortenberg, James Hurley, Andrew Bond, and Kevin Munnelly -- Silicon nanowire biosensor for ultrasensitive and label-free direct detection of miRNAs / Guo-Jun Zhang -- High-throughput and reliable protocols for animal microRNA library cloning / Caide Xiao -- MicroRNA regulation of growth factor receptor signaling in human cancer cells / Keith M. Giles, Andrew Barker, Priscilla M. Zhang, Michael R. Epis, and Peter J. Leedman -- Epigenetic regulation of microRNA expression in cancer / Hani Choudhry and James W.F. Catto -- In vitro functional study of miR-126 in leukemia / Zejuan Li and Jianjun Chen -- Prediction of the biological effect of polymorphisms within microRNA binding sites / Debora Landi, Roberto Barale, Federica Gemignani, and Stefano Landi -- The guideline of the design and validation of miRNA mimics / Zhiguo Wang -- Analysis of targets and functions coregulated by microRNAs / Shu-Jen Chen and Hua-Chien Chen -- Utilization of SSCprofiler to predict a new miRNA gene / Anastasis Oulas and Panayiota Poirazi -- MicroRNA profiling using fluorescence-labeled beads : data acquisition and processing / Thomas Streichert, Benjamin Otto, and Ulrich Lehmann |
| Summary |
The tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes. Recent miRNA studies change the landscape of cancer genetics by scrutinizing the alterations of genome-wide miRNA expressions in most common cancers and their regulatory functions during the development of cancer. The connections between miRNAs and cancer are widespread enough to warrant more comprehensive investigations in the systems biology perspective. In "MicroRNA and Cancer: Methods and Protocols," internationally renowned experts provide the latest miRNA knowledge, the various techniques and methodologies currently available for cancer research application. Authoritative and cutting-edge, this volume serves researchers with the basic principles of experimental and computational methods for microRNA study in cancer research and provides a firm grounding for those who wish to further develop their own applications and tailor them to their own specific research needs |
| Analysis |
neoplasms |
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biomedische wetenschappen |
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biomedicine |
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humane genetica |
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human genetics |
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Medicine (General) |
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Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references at chapter ends, and index |
| Notes |
English |
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Print version record |
| Subject |
Small interfering RNA.
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Cancer.
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Biology.
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Antisense RNA.
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Diseases.
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Life sciences.
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Non-coding RNA.
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RNA.
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MicroRNA.
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Nucleic acids.
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Physical sciences.
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Biology
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RNA, Antisense
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Publication Formats
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Disease
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RNA, Small Untranslated
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Biological Science Disciplines
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RNA, Untranslated
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Antisense Elements (Genetics)
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RNA
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Publication Characteristics
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Neoplasms
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MicroRNAs
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Genetics
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Laboratory Manuals
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Nucleic Acids, Nucleotides, and Nucleosides
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Nucleic Acids
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Natural Science Disciplines
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Chemicals and Drugs
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Disciplines and Occupations
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RNA, Small Interfering
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biology.
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biological sciences.
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physical sciences.
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RNA
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Physical sciences
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Nucleic acids
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Non-coding RNA
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MicroRNA
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Life sciences
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Diseases
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Biology
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|
Antisense RNA
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Cancer
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Small interfering RNA
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Wu, Wei, Dr., editor.
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| ISBN |
9781607618638 |
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160761863X |
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