| Description |
1 online resource (xiv, 530 pages) : illustrations |
| Series |
Methods in molecular medicine ; 51 |
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Methods in molecular medicine ; 51. 1543-1894
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| Contents |
pt. 1. Introduction to the renin-angiotensin system -- Historical perspective to the renin-angiotensin system / J.E. Hall -- Three angiotensin paradigms in one patient: etiology of hypertension, glomerular hemodynamics, and long-term glomerular protection / C.L. Laffer -- pt. 2. Transgenic and gene targeting methods -- Production and use of chimeric mice / J. Takaya -- Genetic manipulation of the renin-angiotensin system using cre-loxP-recombinase / Curt D. Sigmund -- Systemic delivery of a transgene in intact animals by a retroviral vector / M.J. Katovich -- Antisense inhibition of the renin-angiotensin system / D. Mohuczy -- pt. 3. Analysis of transcriptional and translational mechanisms -- Analysis of transcriptional control mechanisms I: techniques for characterization of cis-regulatory elements / C.T. Sherman -- Analysis of transcriptional control mechanisms II: techniques for characterization of trans-acting factors / A.R. Brasier -- Analysis of cytosolic proteins that bind to the 5' leader sequence of the angiotensin AT1 receptor by RNA electromobility shift assay / Z. Wu -- In vitro translation of the angiotensin AT1 receptors in wheat-germ extracts / H. Ji -- pt. 4. Assays for determination of mRNA levels -- Quantitation of angiotensin II receptors by competitive reverse-transcription polymerase chain reaction / W. Wu -- Analysis of RNA by Northern-blot hybridization / P.E. Gallagher -- Use of the ribonuclease protection assay for the analysis and characterization of target mRNAs / S.C. Supowit -- In situ hybridization / S. Riggs Runge -- pt. 5. Assays for determination of protein contents and enzyme activity -- Immunohistochemical detection of angiotensin II receptor / Z.-Q. Wang -- Western blotting / N.R. Madamanchi -- Quantification of angiotensin-converting enzyme (ACE) activity / Q.C. Meng -- Quantification of angiotensin peptides / Q.C. Meng -- Radiolabeling of angiotensin peptides / R.C. Speth -- Assays for radiolabel-photoaffinity labeling of angiotensin receptors / J. Perodin -- Radioligand binding assay / G. Thibault -- Autoradiographic localization and quantification of components of the renin-angiotensin system in tissues / S.Y. Chai -- pt. 6. Biochemical studies of the renin-angiotensin system -- Measurement of intracellular free calcium ion concentration in vascular smooth muscle cells: fluorescence imaging of cytosolic calcium / R.M. Touyz -- Measurement of phospholipase D activation in vascular smooth muscle cells / R.M. Touyz -- Measurement of cylic AMP and cyclic GMP in Xenopus oocytes stimulated with angiotensin II and atrial natriuretic factor / H. Ji -- Measurement of nitric oxide formation / C. Thorup -- Measuring renin secretion from juxtaglomerular cells / A. Kurtz -- pt. 7. Functional studies of the renin-angiotensin system -- Measurement of regional blood flow in the kidney using laser-Doppler flowmetry / R.J. Roman -- Measurement of renal tubular angiotensin II / J.D. Imig -- The in vitro blood-perfused juxtamedullary nephron technique / E.W. Inscho -- In vitro proximal tubule perfusion preparation / J.L. Garvin -- Isolated resistance artery preparation / J.P.M. Wesselman -- Measurement of vascular density / A.S. Greene -- Functional studies of the cerebral circulation / J.H. Lombard |
| Summary |
The renin-angiotensin system has been in the spotlight for more than 100 years because of its vital role in blood pressure regulation, ion and fluid homeostasis, and tissue growth and remodeling. Recent advances in molecular genetic techniques have established that the renin-angiotensin system may well hold the key to the pathogenesis of hypertension, congestive heart failure, chronic renal failure, and stroke. In Angiotensin Protocols, Donna Wang and a panel of hands-on experts describe in step-by-step detail all the major molecular, biochemical, and functional techniques now used in probing this powerful system. These range from the use of embryonic gene targeting to create mutant strains, to the functional investigation of components of the renin-angiotensin system. Following the successful pattern of the Methods in Molecular Biology"!series, each method is crafted for experimental success with detailed instructions, perceptive notes highlighting the most critical steps, and helpful tips on pitfalls to avoid. Comprehensive and highly practical, Angiotensin Protocols translates expert knowledge into readily reproducible methods that enable all biomedical investigators successfully to explore the role of angiotensin in health and disease today |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
Angiotensins -- Laboratory manuals
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Genetics -- Technique.
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Immunoassay.
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Renin-Angiotensin System -- physiology
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Genetic Techniques
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Immunoassay
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Regional Blood Flow -- physiology
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MEDICAL -- Biochemistry.
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Immunoassay
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Genetics -- Technique
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Angiotensins
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Wang, Donna H
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| LC no. |
00027598 |
| ISBN |
9781592590872 |
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159259087X |
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0896037630 |
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9780896037632 |
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