| Description |
1 online resource (streaming video file) (688 secs.) |
| Series |
Biology |
| Summary |
The method presented here describes an assay to follow activation of RhoA specific GDP/GTP Exchange Factors (GEFs) in cultured cells by making use of a mutant RhoA GST fusion protein that has high affinity for activated GEFs. GEFs are precipitated from cell lysates, detected by Western blotting and quantified by densitometry |
| Audience |
For undergraduate, graduate, and professional students |
| Notes |
English |
|
Description based on online resource; title from resource description page |
| Subject |
Biology
|
| Form |
Streaming audio
|
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