| Description |
1 online resource (xv, 344 pages) : illustrations (chiefly color) |
| Series |
Springer protocols. Neuromethods, 1940-6045 ; 123 |
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Neuromethods ; 123. 0893-2336
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Springer protocols (Series) 1949-2448
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| Contents |
Three-dimensional reconstruction of neuronal mitochondria by electron tomography / Guy Perkins -- Measuring mitochondrial shape with ImageJ / Ronald A. Merrill, Kyle H. Flippo, Stefan Strack -- Live imaging mitochondrial transport in neurons / Meredith M. Course, Chung-Han Hsieh, Pei-I Tsai, Jennifer A. Codding-Bui [and others] -- Techniques to investigate bioenergetics of mitochondria / William I. Sivitz -- Respirometry in neurons / Liang Zhang, Eugenia Trushina -- Measuring ATP in axons with FRET / Lauren Y. Shields, Bryce A. Mendelsohn, Ken Nakamura -- Characterizing metabolic states using fluorescence lifetime imaging microscopy (FLIM) of NAD(P)H / Thomas S. Blacker, Michael R. Duchen -- Techniques for simultaneous mitochondrial and cytosolic Ca2+ imaging in neurons / Jacob E. Rysted, Zhihong Lin, Yuriy M. Usachev -- Live imaging of mitochondrial ROS production and dynamic redox balance in neurons / Karolina Can, Sebastian Kügler, Michael Müller -- Monitoring of permeability transition pore openings in isolated individual brain mitochondria / Nickolay Brustovetsky, Tatiana Brustovetsky -- Examination of mitochondrial ion conductance by patch clamp in intact neurons and mitochondrial membrane preparations / Elizabeth A. Jonas, Nelli Mnatsakanyan -- Monitoring of permeability transition pore openings in mitochondria of cultured neurons / Tatiana Brustovetsky, Nickolay Brustovetsky -- Protocols for assessing mitophagy in neuronal cell lines and primary neurons / Ruben K. Dagda Ph. D., Monica Rice -- Examining Mitochondrial Function at Synapses In Situ / Gregory T. Macleod, Maxim V. Ivannikov -- Proteomic analysis of neuronal mitochondria / Kelly L. Stauch, Howard S. Fox -- Measuring mitochondrial pyruvate oxidation / Lawrence R. Gray, Alix A.J. Rouault, Lalita Oonthonpan, Adam J. Rauckhorst [and others] |
| Summary |
"This volume describes a broad spectrum of experimental approaches for investigating structure, function, and transport of neuronal mitochondria in health and disease. Most of these approaches were only recently developed and range from electron tomography-based 3D reconstruction of mitochondrial cristae to patch clamp recording from mitochondria in intact neurons. The chapters in this book cover topics such as mitochondrial proteomics, fluorescence lifetime imaging, respirometry and mitophagy, as well as optical approaches based on the use of genetically engineered fluorescent sensors for monitoring synaptic ATP and axonal ROS generation, mitochondrial Ca2+ cycling and pH changes, and mitochondrial dynamics and axonal trafficking in live neurons. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Techniques to Investigate Mitochondrial Function in Neurons is a valuable and useful resource for a broad range of investigators interested in the function of neuronal mitochondria in health and disease states."--Publisher's description |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from resource home page (Springer, viewed March 21, 2017) |
| Subject |
Mitochondria -- Laboratory manuals
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Neurons -- Physiology -- Laboratory manuals
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Mitochondria -- physiology
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Neurons -- physiology
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Mitochondria -- metabolism
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Neurons -- metabolism
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Neurochemistry -- methods
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Mitochondria
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Neurons -- Physiology
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Strack, Stefan, editor
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Usachev, Yuriy M., editor
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| ISBN |
9781493968909 |
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1493968904 |
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