| Description |
1 online resource (401 p.) |
| Series |
Chromatographic Science Ser |
|
Chromatographic Science Ser
|
| Contents |
Cover -- Half Title -- Series Information -- Title Page -- Copyright Page -- Table of Contents -- Preface -- Contributors -- 1 Introduction to Two-Dimensional Liquid Chromatography -- 1.1 The Basic Concept of Multi-Dimensional Separation -- 1.2 Scope of This Book -- 1.3 Terminology -- 1.4 Why Do We Need More Than One Dimension of Separation? -- 1.5 Types and Modes of 2D-LC Separation -- 1.5.1 Types of 2D Separation -- 1.5.1.1 Serial Coupling of Columns Is Not Two-Dimensional Separation -- 1.5.1.2 Offline vs. Online Separation -- 1.5.1.3 2D-LC Separations With Discontinuous 1D Elution |
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1.5.2 Modes of Online 2D-LC Separation -- 1.5.2.1 Single Heartcut 2D-LC (LC-LC) -- 1.5.2.2 Multiple Heartcut 2D-LC (mLC-LC) -- 1.5.2.3 Selective Comprehensive 2D-LC (sLC×LC) -- 1.5.2.1 Fully Comprehensive 2D-LC (LC×LC) -- 1.6.1 2-For-1 Methods -- 1.6 Scope of Application for 2D-LC in the Context of Analytical Chemistry -- 1.6.2 Methods for Enhancing the Selectivity of 1D-LC -- 1.6.3 Methods for Profiling Complex Materials -- 1.6.4 Methods for Extraction of Chemical Information From Patterns in 2D Chromatograms -- 1.6.5 Methods for Inherently Difficult Analyses |
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1.6.5.1 Desalting And/or Solvent Exchange -- 1.6.5.2 Separations of Analytes With a Large Range in Physicochemical Properties -- 1.6.5.3 Separation of All the D-/L- Enantiomers of a Large Number of Amino Acids -- 1.7 History of 2D-LC Development -- 1.7.1 1970s-1980s: Introduction of Conceptual Frameworks for Multi-Dimensional Separations, and Instrumentation for ... -- 1.7.2 1990s: Automated LC×LC, and the Rise of LC×LC in Proteomics -- 1.7.3 2000s: Development of a Solid Theoretical Foundation -- 1.7.4 2010s: Reduction to Practice -- 1.8 Opportunities and Challenges -- 1.8.1 Challenges |
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1.8.2 Opportunities -- 1.9 Method Development Goals for 2D-LC -- 1.10 Data Analysis -- 1.11 Coupling LC With Other Separation Modes in a 2D Format -- 1.11.1 Coupling With Capillary Electrophoresis -- 1.11.2 Coupling With Supercritical Fluid Chromatography -- 1.11.3 Coupling With Gas Chromatography -- References -- 2 Speed and Performance in Liquid Chromatography -- 2.1 Introduction -- 2.2 Overview of Aspects of Performance Optimization -- 2.3 Impact of Column and Instrument Technologies On Separation Performance -- 2.3.1 Smaller Particles and Higher Pressures -- 2.3.2 Higher Temperatures |
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2.3.3 Superficially Porous Particles (SPPs) -- 2.3.4 Monolithic Columns -- 2.3.5 Non-Porous Particles -- 2.4 Approaches to Optimizing Speed and Performance -- 2.4.1 One-Parameter Optimization -- 2.4.2 Two-Parameter (Poppe) Optimization -- 2.4.2.1 Introduction to Poppe Plots -- 2.4.2.2 An Alternative Way to Compute Poppe Plots -- 2.4.2.3 Long-Time and Short-Time Limits of LC as Revealed By the Poppe Plot -- 2.4.3 Impact of System Parameters On Poppe Plots, the Speed and Limiting Plate Count in LC -- 2.4.3.1 Impact of High Pressure On Speed of LC |
| Notes |
Description based upon print version of record |
|
2.4.3.2 Impact of Particle Size On the Speed of LC |
| Form |
Electronic book
|
| Author |
Carr, Peter W
|
| ISBN |
9781000817331 |
|
1000817334 |
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