| Description |
1 online resource (1 volume) : illustrations |
| Series |
Methods in molecular biology ; v. 255 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 255
|
| Contents |
BAC library construction / K. Osoegawa and P.J. de Jong -- Construction of small genome BAC library for functional and genomic applications / D. Smajs, S.J. Norris and G.M. Weinstock -- Preparation of BAC libraries from bacterial genomes by in vitro packaging / S. Pal, S. Mak and G.M. Weinstock -- Exploring transformation-associated recombination cloning for selective isolation of genomic regions / N. Kouprina [and others] -- Purification of BAC DNA / T.S. Poulsen -- Hybridization-based selection of BAC clones / C.S. Lim and U.J. Kim -- Applications of interspersed repeat sequence polymerase chain reaction / H. Zimdahl [and others] -- BAC mapping using fluorescence in situ hybridization / X.N. Chen and J.R. Korenberg -- High-throughput BAC fingerprinting / J. Schein [and others] -- BAC end sequencing / T.S. Poulsen and H.E. Johnsen -- Radiation hybrid mapping with BAC ends / M. Olivier, S. Brady and D.R. Cox -- Shotgun library construction for DNA sequencing / B.A. Roe -- Rolling circle amplification for sequencing templates / P.F. Predki [and others] -- Transposon-mediated sequencing / R. Reeg and A. Madan -- Pyrosequencing: A tool for DNA sequencing analysis / E. Elahi and M. Ronaghi -- Use of fimers to eliminate polymerase chain reaction and primer-dimer artifacts and to increase yield in bac-sequencing reactions / A. Malykh [and others] -- Retrofitting bacs and pacs with loxP transposons to generate nested deletions / P.K. Chatterjee -- Preparing nested-deletion template DNA for field inversion gel electrophoresis analyses and position-specific end sequencing with transposon primers / P.K. Chatterjee and J.C. Baker, Jr. -- BAC finishing strategies / C. Bird and D. Grafham -- Using the TIGR assembler in shotgun sequencing projects / M. Pop and D. Kosack -- Finishing "working draft" BAC projects by directed sequencing with thermofidelase and fimers / A. Malykh [and others] -- Optimized multiplex polymerase chain reaction: An effective method for rapid gap closure / D. Radune and H. Tettelin -- Assembly of DNA sequencing data / J. Schmutz, J. Grimwood and R.M. Myers -- Sequence finishing / J. Schmutz, J. Grimwood and R.M. Myers -- Quality assessment of finished BAC sequences / J. Schmutz, J. Grimwood and R.M. Myers |
| Summary |
The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes |
| Analysis |
bacteria |
|
chromosomen |
|
chromosomes |
|
dna sequencing |
|
Nucleic Acids |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Bacterial artificial chromosomes -- Laboratory manuals
|
|
Bacterial artificial chromosomes.
|
|
Gene mapping.
|
|
Molecular cloning.
|
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Gene libraries.
|
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Chromosomes, Artificial, Bacterial
|
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Chromosome Mapping
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Cloning, Molecular
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Genomic Library
|
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SCIENCE -- Biotechnology.
|
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Molecular cloning
|
|
Gene mapping
|
|
Gene libraries
|
|
Bacterial artificial chromosomes
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Zhao, Shaying
|
|
Stodolsky, Marvin
|
| ISBN |
1592597521 |
|
9781592597529 |
|
0896039889 |
|
9780896039889 |
|
