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Author Rustad, Mark

Title Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description online resource (streaming video file)(693 seconds)
Series Bioengineering
Summary A new generation of cell-free transcription-translation platforms has been engineered to construct biochemical systems in vitro through the execution of gene circuits. In this article, we describe how bacteriophages, such as MS2, ΦΧ174, and T7, are synthesized from their genome using an all E. coli cell-free TXTL system
Notes Title from resource description page
Audience For undergraduate, graduate, and professional students
Notes English
Subject Bioengineering
Form Streaming video