| Description |
v, 800 pages ; 29 cm |
| Contents |
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies |
| Notes |
Includes index |
| Subject |
Molecular cloning -- Laboratory manuals.
|
| Genre/Form |
Laboratory manuals.
|
| Author |
Russell, David W. (David William), 1957-
|
| LC no. |
2005022077 |
| ISBN |
0879697725 hardcover alkaline paper |
|
0879697717 paperback alkaline paper |
|
