| Description |
1 online resource (1 volume) : color illustrations |
| Contents |
2.2 Lipid extraction from biological samples -- 2.3 Solid-phase extraction for sample enrichment -- 2.4 Derivatisation approaches for lipid analysis -- 3. Direct infusion and chromatography-based approaches for lipid analysis -- 3.1 Thin-layer chromatography -- 3.2 Gas chromatography -- 3.3 High-performance and ultra-high-pressure liquid chromatography -- 3.3.1 Column retention mechanisms and solvent system -- 3.4 Supercritical fluid chromatography -- 4. Quantitation of lipid levels -- 4.1 Quality control checks -- 5. Mass spectrometry for lipid analysis -- 5.1 Ionisation sources -- 5.2 Mass analysers -- 5.2.1 Fourier transform ion cyclotron resonance and orbitrap -- 5.2.2 Quadrupole -- 5.2.3 Time of flight -- 5.3 Fragmentation techniques used in lipid analysis -- 5.3.1 Electron-based dissociation -- 5.3.2 Photodissociation -- 5.4 Ion mobility -- 5.5 Mass spectrometry imaging -- 6. LC-MS data processing and future directions -- References -- 3 -- Fatty acid and phospholipid biosynthesis in prokaryotes -- List of abbreviations -- 1. Overview of bacterial lipid metabolism -- 2. Membrane systems of bacteria -- 3. The initiation module -- 3.1 Acyl carrier protein -- 3.2 Acetyl-coenzyme A carboxylase -- 3.3 Malonyl transacylase -- 3.4 3-ketoacyl-acyl carrier protein synthase III -- 3.5 Regulation in the initiation module -- 4. The elongation module -- 4.1 3-ketoacyl-acyl carrier protein synthases I and II -- 4.2 3-ketoacyl-acyl carrier protein reductase -- 4.3 3-hydroxyacyl-acyl carrier protein dehydratases -- 4.4 Enoyl-acyl carrier protein reductase -- 4.5 Regulation in the elongation module -- 4.6 Bacteria with type I fatty acid synthase -- 5. The acyltransfer module -- 5.1 The PlsB/PlsC system -- 5.2 The PlsX/PlsY/PlsC system -- 5.3 Regulation in the acyltransfer module -- 5.4 Use of extracellular fatty acids -- 5.4.1 Acyl-coenzyme A synthetase |
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5.4.2 Acyl-acyl carrier protein synthetase -- 5.4.3 Fatty acid kinase -- 5.5 Thioesterases -- 5.6 Fatty acids as a carbon source -- 6. The phospholipid module -- 6.1 Phosphatidate cytidylyltransferase -- 6.2 Phosphatidylethanolamine production -- 6.2.1 Phosphatidylserine synthase -- 6.3 Phosphatidylserine decarboxylase -- 6.4 Phosphatidylglycerol synthesis -- 6.4.1 Phosphatidylglycerolphosphate synthase -- 6.4.2 Phosphatidylglycerolphosphate phosphatases -- 6.5 Cardiolipin biosynthesis -- 6.6 Use of phospholipid headgroups -- 6.6.1 Use of phospholipid fatty acids -- 6.7 Modification of phospholipids -- 6.7.1 Origin of cyclopropane fatty acids -- 6.7.2 Phospholipid desaturases -- 6.7.3 Modification of polar headgroups -- 6.7.4 Phospholipases -- 6.8 Phospholipid diversity in bacteria -- 6.9 Membrane lipids lacking phosphorus -- 6.10 Regulation in the phospholipid module -- 7. Genetic regulation of lipid metabolism -- 7.1 Gram-negative bacteria -- 7.1.1 Coordination of fatty acid synthesis and degradation by FadR -- 7.1.2 DesT/FabR control of unsaturated fatty acid synthesis -- 7.2 Gram-positive bacteria -- 7.2.1 The FapR system -- 7.2.2 The FabT system -- 7.3 Stress response regulators -- 7.3.1 Two-component systems -- 7.3.2 Alternate sigma factors -- 8. Future directions -- References -- 4 -- Lipid metabolism in plants -- List of abbreviations -- 1. Introduction -- 2. Plant lipid geography -- 2.1 Plastids -- 2.2 Endoplasmic reticulum and lipid bodies -- 2.3 Mitochondria -- 2.4 Peroxisomes and glyoxysomes -- 3. Acyl-acyl carrier protein synthesis in plants -- 3.1 Components of plant fatty acid synthase -- 3.2 The first double bond is introduced by soluble acyl-acyl carrier protein desaturases -- 3.3 Acyl-acyl carrier protein thioesterases release fatty acids for export -- 4. Acetyl-coenzyme A carboxylase and control of fatty acid synthesis |
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4.1 Most plants have two acetyl-coenzyme A carboxylases -- 4.2 Acetyl-coenzyme A carboxylase is a control point for fatty acid synthesis -- 5. Phosphatidic acid synthesis occurs via prokaryotic and eukaryotic acyltransferases -- 5.1 Plastidial acyltransferases use acyl-acyl carrier protein substrates -- 5.2 Extraplastidial acyltransferases use acyl-coenzyme A substrates -- 5.3 The 16:3 and 18:3 plants have different proportions of prokaryotic flux -- 6. Membrane glycerolipid synthesis -- 6.1 Lipid trafficking between plastids and endomembranes -- 6.2 Glycerolipids are substrates for polyunsaturated fatty acid synthesis -- 6.3 Some plants use endoplasmic reticulum glycerolipids as substrates for production of unusual fatty acids -- 7. Lipid storage in plants -- 7.1 Lipid body structure and biogenesis -- 7.2 The pathways of triacylglycerol biosynthesis -- 7.3 Control of triacylglycerol yield -- 7.4 Control of triacylglycerol composition -- 7.5 Triacylglycerols in vegetative tissues -- 7.6 Triacylglycerol engineering: some case studies -- 8. Protective lipids: cutin, waxes, suberin and sporopollenin -- 8.1 Fatty acid elongation and wax production -- 9. Sphingolipid biosynthesis -- 10. Oxylipins as plant hormones -- 11. Sterol and isoprenoid biosynthesis -- 12. Future prospects -- Acknowledgements -- References -- 5 -- Fatty acid handling in mammalian cells -- List of abbreviations -- 1. Introduction -- 2. Fatty acid biosynthesis -- 2.1 Acyl-CoA carboxylase -- 2.1.1 Regulation of ACC by allosteric mechanisms and phosphorylation -- 2.1.2 Transcriptional regulation -- 2.2 Cytosolic fatty acid synthase -- 2.3 Mitochondrial fatty acid synthase -- 3. Fatty acid uptake, activation and trafficking -- 3.1 CD36 -- 3.2 Fatty acid transport proteins/acyl-CoA synthetase very long chain -- 3.3 Acyl-CoA synthetases -- 3.4 Fatty acid-binding proteins |
| Notes |
Includes index |
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Print version record |
| Subject |
Lipids -- Metabolism.
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Lipoproteins -- Metabolism
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Membranes (Biology) -- Metabolism
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Lipid Metabolism
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Lipoproteins -- metabolism
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Lipids -- Metabolism
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Lipoproteins -- Metabolism
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| Form |
Electronic book
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| Author |
Ridgway, Neale David, editor.
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McLeod, Roger S., editor.
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| ISBN |
9780323983280 |
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0323983286 |
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