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Book Cover
E-book
Author Ober, Raimund J

Title Quantitative Bioimaging An Introduction to Biology, Instrumentation, Experiments, and Data Analysis for Scientists and Engineers
Published Milton : Taylor & Francis Group, 2020
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Description 1 online resource (552 p.)
Contents Cover -- Half Title -- Title Page -- Copyright Page -- Dedication -- Table of Contents -- Preface -- Acknowledgments -- I Introduction -- Overview -- 1 Then and Now -- 2 Introduction to Two Problems in Cellular Biology -- 2.1 Antibody trafficking -- 2.2 Localization experiments -- 2.3 Association experiments -- 2.4 Dynamic studies -- 2.5 Iron transport, transferrin, and the transferrin receptor -- 3 Basics of Microscopy Techniques -- 3.1 Optical microscopy for cell biology -- 3.2 Transmitted light microscopy -- 3.3 Fluorescence microscopy -- 3.3.1 Fluorescence
3.3.2 Layout of an epifluorescence widefield microscope -- 3.4 Inverted versus upright microscope -- 3.5 Components of commercial microscopes -- 3.5.1 Light sources -- 3.5.2 Objectives -- 3.6 Fixed and live cell experiments -- 3.7 Sample preparation -- 3.8 A note regarding safety -- 4 Introduction to Image Formation and Analysis -- 4.1 Image formation and point spread functions -- 4.2 Resolution: an elementary introduction -- 4.3 Modeling and analyzing the data -- Notes -- Exercises -- II Biology and Chemistry -- Overview -- 5 From genes to proteins -- 5.1 Bonds -- 5.2 DNA and genes
5.3 How are proteins made? -- 5.4 Structures of proteins -- 5.5 Protein structure determination -- 6 Antibodies -- 6.1 Structure of antibodies -- 6.2 Variable regions and binding activity -- 6.3 Constant regions -- 6.4 Antibody production for laboratory and clinical use -- 6.4.1 The classical method: hybridoma technology -- 6.5 Diagnostic techniques using antibody detection methods -- 6.5.1 Enzyme-linked immunosorbent assay -- 6.5.2 Surface plasmon resonance for the quantitation of the affinity of an interaction -- 7 Cloning of genes for protein expression -- 7.1 Features of expression constructs
7.2 Methods for generating expression plasmids -- 7.2.1 Restriction enzymes -- 7.2.2 Polymerase chain reaction -- 7.2.3 Details of approaches for generating expression plasmids -- 7.2.4 Transfection of mammalian cells for expression -- 7.3 Antibody engineering -- 7.3.1 Chimeric antibodies -- 7.3.2 Humanized antibodies -- 7.3.3 Isolation of V regions -- 8 Principles of Fluorescence -- 8.1 Wave and particle description of light -- 8.2 Jablonski diagram -- 8.3 Stokes shift -- 8.4 Photobleaching -- 8.5 Photophysical characterization of fluorophores -- 8.5.1 Quantum yield
8.5.2 Beer-Lambert law, effective absorption cross section and molar extinction coefficient -- 8.5.3 Brightness of a fluorophore -- 8.6 Excitation and emission spectra -- 8.7 Fluorophores -- 8.7.1 Chemical fluorescent dyes -- 8.7.1.1 Labeling of proteins via cysteine or lysine residues -- 8.7.1.2 Labeling of proteins with fluorophore-conjugated streptavidin -- 8.7.1.3 In situ labeling of proteins in cells using peptide tags -- 8.7.2 Quantum dots -- 8.7.2.1 Labeling of proteins with quantum dots -- 8.7.3 Fluorescent proteins -- 8.7.4 Photoactivatable and photoswitchable fluorescent probes
Notes 8.7.5 Other labeling modalities
Description based upon print version of record
Form Electronic book
Author Chao, Jerry
Ward, E. Sally
ISBN 0429892128
9780429892127