Title Single domain antibodies : methods and protocols / edited by Dirk Saerens and Serge Muyldermans

Published New York : Humana Press, ©2012

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Description 1 online resource (xv, 580 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 911 Methods in molecular biology (Clifton, N.J.) ; 911. 1064-3745 Contents From whole monoclonal antibodies to single domain antibodies : think small / Jean-Luc Teillaud -- Introduction to heavy chain antibodies and derived nanobodies / Cécile Vincke and Serge Muyldermans -- Overview and discovery of IgNARs and generation of VNARs / Stewart D. Nuttall -- Creation of the large and highly functional synthetic repertoire of human VH and Vk domain antibodies / Olga Ignatovich [and others] -- Preparation of a naive library of camelid single domain antibodies / Aurelien Olichon and Ario de Marco -- Selection by phage display of single domain antibodies specific to antigens in their native conformation / Peter Verheesen and Toon Laeremans -- Semiautomated panning of naive camelidae libraries and selection of single-domain antibodies against peptide antigens / Jyothi Kumaran, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi -- Pichia surface display : a tool for screening single domain antibodies / Kristof De Schutter and Nico Callewaert -- Bacterial two hybrid : a versatile one-step intracellular selection method / Mireille Pellis, Serge Muyldermans, and Cécile Vincke -- Intracellular antibody capture (IAC) methods for single domain antibodies / Tomoyuki Tanaka and Terence H. Rabbitts -- Selection of functional single domain antibody fragments for interfering with protein-protein interactions inside cells : a 'one plasmid' mammalian two-hybrid system / Tomoyuki Tanaka and Terence H. Rabbitts -- Cell-free selection of domain antibodies by in vitro compartmentalization / Armin Sepp and Andrew Griffiths -- Selection of VHHs under application conditions / Edward Dolk, Theo Verrips, and Hans de Haard -- Isolation and characterization of Clostridium difficile toxin-specific single-domain antibodies / Greg Hussack [and others] -- Selection of VHH antibody fragments that recognize different Ab depositions using complex immune libraries / Rinse Klooster, Kim S. Rutgers, and Silvere M. van der Maarel -- Expression of single-domain antibodies in bacterial systems / Toya Nath Baral and Mehdi Arbabi-Ghahroudi -- Expression of VHHs in Saccharomyces cerevisiae / Andrea Gorlani, Hans de Haard, and Theo Verrips -- Stable expression of chimeric heavy chain antibodies in CHO cells / Vishal Agrawal [and others] -- Production of Camel-Like Antibodies in Plants / Sylvie De Buck [and others] -- Selecting and purifying autonomous human variable heavy (VH) domains / Raffi Tonikian and Sachdev S. Sidhu -- Solubility and stability engineering of human VH domains / Dae Young Kim, Wen Ding, and Jamshid Tanha -- Improvement of proteolytic stability through in silico engineering / Lucy Rutten, Hans de Haard, and Theo Verrips -- Selection of human VH single domains with improved biophysical properties by phage display / Kip Dudgeon [and others] -- Improvement of single domain antibody stability by disulfide bond introduction / Yoshihisa Hagihara and Dirk Saerens -- Characterization of single-domain antibodies with an engineered disulfide bond / Greg Hussack, C. Roger MacKenzie, and Jamshid Tanha -- Affinity maturation of single-domain antibodies by yeast surface display / Akiko Koide and Shohei Koide -- Multivalent display of single-domain antibodies / Jianbing Zhang and C. Roger MacKenzie -- Methods for determining the PK parameters of AlbudAbs[TM] and of long serum half-life drugs made using the AlbudAb[TM] technology / Daniel Rycroft and Lucy J. Holt -- Fluorescent protein specific nanotraps to study protein-protein interactions and histone-tail peptide binding / Garwin Pichler, Heinrich Leonhardt, and Ulrich Rothbauer -- Site-specific labeling of his-tagged nanobodies with 99mTc : a practical guide / Catarina Xavier [and others] -- Nanobody-based chromatin immunoprecipitation / Trong Nguyen Duc [and others] -- User-friendly expression plasmids enable the fusion of VHHs to application-specific tags / Ario de Marco -- Application of single-domain antibodies in tumor histochemistry / Kien T. Maik and C. Roger MacKenzie -- Nanobodies as structural probes of protein misfolding and fibril formation / Erwin De Genst and Christopher M. Dobson -- Molecular imaging using nanobodies : a case study / Nick Devoogdt [and others] -- Case study on live cell apoptosis-assay using lamin-chromobody cell-lines for high-content analysis / Kourosh Zolghadr [and others] Summary The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications. In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Analysis Medicine Immunology Monoclonal antibodies Biomedicine Antibodies biomedische wetenschappen immunologie antilichamen Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject Monoclonal antibodies -- Laboratory manuals Monoclonal antibodies. Antibodies, Monoclonal Monoclonal antibodies Biology. Health & Biological Sciences. Microbiology & Immunology. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Saerens, Dirk. Muyldermans, Serge. ISBN 9781617799686 1617799688 161779967X 9781617799679

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