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Title Phytoplasmas : methods and protocols / edited by Rita Musetti and Laura Pagliari
Published New York : Humana Press : Springer, [2018]


Description 1 online resource (xi, 362 pages) : illustrations (some color)
Series Methods in molecular biology ; 1875
Methods in molecular biology (Clifton, N.J.) ; v. 1875. 1064-3745
Contents Phytoplasmas : an introduction / L. Pagliari and R. Musetti -- Micro-tom tomato grafting for stolbur-phytoplasma transmission : different grafting techniques / Sara Buoso and Alberto Loschi -- Phytoplasma transmission : insect rearing and infection protocols / L. Pagliari, J. Chuche, D. Bosco, and D. Thiery -- Sampling methods for leafhopper, planthopper, and psyllid vectors / Kerstin Kruger and Nicola Fiore -- Symptoms of phytoplasma diseases / Paolo Ermacora and Ruggero Osler -- Comparison of different procedures for DNA extraction for routine diagnosis of phytoplasmas / Carmine Marcone -- Standard detection protocol : PCR and RFLP analyses based on 16S rRNA gene / Assunta Bertaccini, Samanta Paltrinieri, and Nicoletta Contaldo -- PCR-based sequence analysis on multiple genes other than 16S rRNA gene for differentiation of phytoplasmas / Marta Martini, Kristi D. Bottner-Parker, and Ing-Ming Lee -- Real-time PCR protocol for phytoplasma detection and quantification / Yusuf Abou-Jawdah, Vicken Aknadibossian, Maan Jawhari, Patil Tawidian, and Peter Abrahamian -- Duplex TaqMan real-time PCR for rapid quantitative analysis of a phytoplasma in its host plant without external standard curves / Sanja Baric -- Multiplex-PCR method for diagnosis of AY-group phytoplasmas / Shigeyuki Kakizawa -- One-step multiplex quantitative RT-PCR for the simultaneous detection of viroids and phytoplasmas / Ioanna Malandraki, Christina Varveri, and Nikon Vassilakos -- Rapid protocol of crude RNA/DNA extraction for RT-qPCR detection and quantification / Claudio Ratti, Stefano Minguzzi, and Massimo Turina -- Quantitative analysis with droplet digital PCR / Natasa Mehle and Tanja Dreo -- Rapid sample preparation and LAMP for phytoplasma Detection / Jennifer Hodgetts -- Assembly of phytoplasma genome drafts from illumina reads using phytoassembly / Cesare Polano and Giuseppe Firrao -- Protocol for the definition of a multi-spectral sensor for specific foliar disease detection : case of "Flavescence Doree" / H. Al-Saddik, A. Laybros, J.C. Simon, and F. Cointault -- Transcriptomic analyses of phytoplasmas / Davide Pacifico, Simona Abba, and Sabrina Palmano -- Sieve elements : the favourite habitat of phytoplasmas/ Aart J.E. van Bel -- Laser microdissection of phytoplasma-infected grapevine leaf phloem tissue for gene expression study / Simonetta Santi -- Collection of phloem sap in phytoplasma-infected plants / Matthias R. Zimmermann, Torsten Knauer, and Alexandra C.U. Furch -- DAPI and confocal laser-scanning microscopy for in vivo imaging of phytoplasmas / Rita Musetti and Stefanie Vera Buxa -- Immunofluorescence assay to study early events of vector salivary gland colonization by phytoplasmas / Luciana Galetto, Marta Vallino, Mahnaz Rashidi, and Cristina Marzachı -- Characterization of phytoplasmal effector protein interaction with proteinaceous plant host targets using bimolecular fluorescence complementation (BiFC) / Katrin Janik, Hagen Stellmach, Cecilia Mittelberger, and Bettina Hause -- Collection, identification, and statistical analysis of volatile organic compound patterns emitted by phytoplasma infected plants / Jurgen Gross, Jannicke Gallinger, and Margit Rid -- Quantification of phytohormones by HPLC-MS/MS including phytoplasma-infected plants / Marilia Almeida-Trapp and Axel Mithofer
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink viewed October 26, 2018)
Subject Phytoplasmas -- Laboratory manuals
Laboratory Manuals
Botany & plant sciences.
Science -- Life Sciences -- Botany.
Genre/Form Electronic books
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Musetti, Rita, editor
Pagliari, Laura, editor
ISBN 9781493988372