| Description |
1 online resource (xvi, 177 pages) : illustrations (some color) |
| Contents |
Probes and Tags to Study Biomolecular Function; Contents; Preface; List of Contributors; 1 Fluorescent Sterols for the Study of Cholesterol Trafficking in Living Cells; 2 Lipid Binding Proteins to Study Localization of Phosphoinositides; 3 The Use of Lipid-Binding Toxins to Study the Distribution and Dynamics of Sphingolipids and Cholesterol; 4 "FlAsH" Protein Labeling; 5 AGT/SNAP-Tag: A Versatile Tag for Covalent Protein Labeling; 6 Trimethoprim Derivatives for Labeling Dihydrofolate Reductase Fusion Proteins in Living Mammalian Cells |
| Summary |
The only resource to systematically review current experimental methods, this handy reference enables researchers to select the best solution for their experimental problems. For each method covered, the book provides step-by-step protocols, illustrated by typical research applications. After an initial section on probing the lipid bilayer, the text moves on to discuss probing proteins -- including membrane proteins -- and nucleic acids. The first single publication to incorporate chemical markers, fluorescent probes and genetic tags allows a well-informed comparison of different solutions for |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Molecular Probes.
|
|
Molecular Biology.
|
|
Molecular Probe Techniques.
|
|
Molecular biology -- Research -- Methodology.
|
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Molecular probes.
|
| Form |
Electronic book
|
| Author |
Miller, Lawrence W.
|
|
Wiley InterScience (Online service)
|
| ISBN |
1281947113 |
|
3527623094 |
|
3527623108 (electronic bk.) |
|
9781281947116 |
|
9783527315666 |
|
9783527623099 |
|
9783527623105 (electronic bk.) |
|
(Cloth) |
|
