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Book Cover
E-book
Author Melter, Oto

Title Principles and Practicals in Medical Microbiology
Published Prague : Karolinum Press, 2014

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Description 1 online resource (135 pages)
Contents Cover; Contents; Introduction; 1 Laboratory safety rules; 1.1 Purpose of the safety principles; 1.2 Principles; 2 Specimen collection & diagnostic principles; 2.1 Specimen collection & transport; 2.2 Material & methods; 2.3 Conditions for specimen collection & transport; 2.4 Request form; 2.5 Diagnostic principles in medical microbiology; 2.6 Light microscopy; 2.7 Gram staining -- basic staining procedure in clinical microbiology; 2.7.1 Smear preparation; 2.7.2 Quality of cell wall & Gram staining procedure; 2.8 Practical part -- specimen collection & diagnostic principles; 2.9 Lab quiz
3 Direct detection & typing of infectious agents3.1 Definition of agent detection & typing; 3.2 Application of microscopy technics; 3.3 Application of nucleic acid based methods; 3.3.1 Principle of DNA amplification by PCR methods; 3.3.2 Principle of DNA hybridization methods; 3.3.3 Principle of sequence based methods; 3.4 Other methods in direct antigen detection; 3.4.1 Immunochromatographic method; 3.5 Practical part -- direct detection & typing of infectious agents; 3.6 Lab quiz; 4 Cultivation of infectious agents; 4.1 Cultivation -- definition
4.2 Cultivation of bacterial, viral & parasitic agents4.3 Media for bacterial cultivation; 4.4 Examples of bacterial cultures; 4.5 Conditions for cultivation; 4.6 Special staining procedures; 4.7 P ractical part -- special stainings & cultivation; 4.8 Lab quiz; 5 Antibiotic susceptibility testing; 5.1 Antimicrobials; mode of action & resistance; 5.2 Antimicrobial susceptibility testing; 5.3 Basic methods; 5.4 Interpretations of the results; 5.5 Other methods; procedures & interpretation; 5.6 Practical part -- special stainings & cultivation of infectious agents; 5.7 Lab quiz
6 Identification of infectious agents6.1 Definition of identification of infectious agents; 6.2 Phenotypical methods; 6.3 Application of phenotypical methods; 6.4 Genotypical methods & application; 6.5 Practical part -- identification of infectious agents; 6.6 Lab quiz; 7 Detection of specific antibodies; 7.1 Immune response; 7.2 Antigens & antibodies; 7.3 Basic serological methods; 7.4 Serological methods & application; 7.5 Practical part -- detection of specific antibodies; 7.6 Lab quiz; 8 General mycology; 8.1 Fungi; 8.2 Classification; 8.3 Diagnostics; 8.3.1 Microscopy; 8.3.2 Cultivation
8.3.3 Identification8.4 Susceptibility to antimycotical drugs; 8.5 Serological methods; 8.6 Direct detection of the agent in clinical material; 8.7 Practical part -- general mycology; 8.8 Lab quiz; 9 General virology; 9.1 Definition of viruses; 9.2 History; 9.3 Electronmicroscopic evidence; 9.4 Virus detection by other methods; 9.5 Praktical part -- general virology; 9.6 Lab quiz; 10 General parasitology; 10.1 Parasitism, parasite and host; 10.2 Classification; 10.3 Diagnostic methods; 10.4 Material and diagnostics; 10.5 Practical part -- general parasitology; 10.6 Lab quiz
Notes 11 Molecular microbiology & epidemiology
Print version record
Subject Medical microbiology.
Microbiological techniques
Medical microbiology
Form Electronic book
Author Malmgren, Annika
ISBN 9788024625447
802462544X
8024624133
9788024624136