| Description |
x, 454 pages : illustrations ; 25 cm |
| Contents |
1. Real-Time PCR: History and Fluorogenic Chemistries / Ian M. Mackay, John F. Mackay, Michael D. Nissen, and Theo P. Sloots -- 2. Oligonucleotide Design for In-House Real-Time PCR Applications in Microbiology / Andreas Nitsche -- 3. QPCR: Target Preparation / Tania Nolan, Reinhold Mueller and Stephen Bustin -- 4. Standards and Controls: Concepts for Preparation and Use in Real-Time PCR Applications / Amy Muska, Edith Peck and Stuart Palmer -- 5. Quantification of Micro-Organisms: Not Human, Not Simple, Not Quick / Ian M. Mackay, Stephen A. Bustin, José Manuel Andrade, Mikael Kubista and Theo P Sloots -- 6. Multiplex rtPCR in Microbiology / Nick M. Cirino, Norma P. Tavakoli, Susan Madison-Antenucci and Christina Egan -- 7. The Role of Real-time PCR in Routine Microbial Diagnostics / Eric C.J. Claas, Willem J.G. Melchers and Adriaan J.C. van den Brule -- 8. Challenges Facing Real-Time PCR Characteristaion of Acute Respiratory Tract Infections / Ian M. Mackay, Katherine E. Arden, Michael D. Nissen and Theo P Sloots -- 9. Rapid Detection of Bioterror Agents / Andreas Nitsche -- 10. Experts Roundtable: Real-Time PCR and Microbiology / M.G.H.M. Beld, et al |
| Summary |
"Real-time PCR has established itself as a sensitive and specific qualitative and quantitative technique that has become important to all areas of microbiology. This book describes and explains some of the more complex aspects of real-time PCR presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardisation, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion providing an insightful, topical and interesting discourse with contributions from over 30 authorities and experts on real-time PCR." -- Publisher's website |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Medical microbiology.
|
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Molecular diagnosis.
|
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Microbiology -- Technique.
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Polymerase chain reaction.
|
| Author |
Mackay, Ian M. (Ian Maxwell)
|
| LC no. |
2007299793 |
| ISBN |
9781904455189 |
|
