| Description |
vii, 284 p., [4] p. of plates : ill. ; 25 cm |
| Contents |
Machine generated contents note: 1.An Introduction to Real-Time PCR / Nick A. Saunders -- 2.An Overview of PCR Platforms / Julie M. J. Logan / Kirstin J. Edwards -- 3.Homogeneous Fluorescent Chemistries for Real-Time PCR / Dario L. Leslie / Martin A. Lee / David J. Squirrell / Tom Brown -- 4.Reference Gene Validation Software for Improved Normalization / Michael W. Pfaffl / Jo Vandesompele / Mikael Kubista -- 5.Data Analysis Software / Michael W. Pfaffl / Mikael Kubista / Jo Vandesompele -- 6.Performing Real-time PCR / Julie M. J. Logan / Kirstin J. Edwards -- 7.Internal and External Controls for Reagent Validation / David J. Squirrell / Dario L. Leslie / Martin A. Lee -- 8.Introduction to the Applications of Real-Time PCR / Nick A. Saunders -- 9.Analysis of mRNA Expression by Real-time PCR / Tania Nolan / Stephen A. Bustin -- 10.Validation of Array Data / Elisa Wurmbach -- 11.Mutation Detection by Real-Time PCR / Rong Mao / Elaine Lyon / Jeffrey Swensen -- |
|
Contents note continued: 12.Real-Time NASBA / Catherine Moore / Julie D. Fox / Diana Westmoreland -- 13.Applications in Clinical Microbiology / Andrew David Sails -- 14.Diagnosis of Invasive Fungal Infections / David S. Perlin -- 15.Biodefence / Nick M. Cirino / Kimberlee A. Musser / Christina Egan -- 16.Real-time PCR: Application to Food Authenticity and Legislation / Gordon Wiseman -- 17.Molecular Haplotyping by Real-time PCR / Alison Millson / Genevieve Pont-Kingdon / Elaine Lyon |
| Summary |
"This essential manual presents a comprehensive guide to the most up-to-date technologies and applications as well as providing an overview of the theory of this increasingly important technique. Renowned experts in the field describe and discuss the latest PCR platforms, fluorescent chemistries, validation software, data analysis, and internal and external controls. This timely and authoritative volume also discusses a wide range of RT-PCR applications including: clinical diagnostics, biodefense, RNA expression studies, validation of array data, mutation detection, food authenticity and legislation, NASBA, molecular halotyping, and much more. An essential book for all laboratories using PCR." --Book Jacket |
| Notes |
Formerly CIP. Uk |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Polymerase Chain Reaction -- methods
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Polymerase Chain Reaction -- methods
|
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Polymerase chain reaction
|
| Genre/Form |
Aufsatzsammlung
|
| Author |
Logan, Julie (Julie M. J.)
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Edwards, Kirstin (Kirstin J.)
|
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Saunders, Nick (Nick A.)
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| LC no. |
2009419187 |
| ISBN |
1904455395 (hbk.) : |
|
9781904455394 (hbk.) : |
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