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Author Lemcke, Heiko

Title Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (386 secs.)
Series Biology
Summary Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the intercellular transfer of small RNAs in real time, with high spatio-temporal resolution
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Biology
Form Streaming audio