| Description |
1 online resource (289 pages) |
| Contents |
Contributors; Preface; Ch 01: Homogeneous Fluorescent Chemistries for Real-time PCR; Ch 02: Internal and Other Controls for Real-time PCR Validation; Ch 03: Analysis of mRNA Expression by Real-time PCR; Ch 04: Detection of Biothreats by Real-time PCR; Ch 05: Veterinary Applications of Real-time PCR for Detection and Diagnosis of Infectious Agents; Ch 06: Applications in Clinical Microbiology; Ch 07: The Extraction and Purification of Nucleic Acids for Analysis by PCR |
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Ch 08: Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase or Decrease the Specificity of qPCRCh 09: Real-time PCR Arrays; Ch 10: The Validation of Real-time PCR Assays for Infectious Diseases; Ch 11: MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay; Ch 12: Management Aspects of Real-time PCR-based Assay Development, Validation, Verification and Implementation; Index |
| Summary |
Real-time PCR technology is an established powerful research tool used in many scientific disciplines and is also utilized for mainstream testing in the regulated markets, such as food, veterinary, and human in-vitro diagnostics. This essential manual provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications, and it provides an overview of the theory of this increasingly important technique. Renowned international authors present detailed technical insights into the underlying principles, methods, and practice of real-time PCR. The initial chapters cover the important aspects of real-time PCR, including choosing an instrument and probe system, set-up, nucleic acid synthesis, sample extraction controls, and validation and data analysis. Further chapters provide a comprehensive overview of important real-time PCR methodologies, such as quantification, expression analysis, and mutation detection. This is complemented by the final chapters, which address the application of real-time PCR to diagnosis of infectious diseases, biodefense, veterinary science, food authenticity, and molecular haplotyping. This timely and authoritative volume serves both as a basic introduction to real-time PCR and as a source of current trends and applications for those already familiar with the technology. The editors also aim to stimulate readers of all levels to develop their own innovative approaches to real-time PCR. The book will be an essential text for all laboratories using PCR |
| Notes |
Print version record |
| Subject |
Polymerase chain reaction.
|
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Molecular biology -- Technique.
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Polymerase Chain Reaction
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Molecular biology -- Technique
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Polymerase chain reaction
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| Form |
Electronic book
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| Author |
Lee, Martin A. (Martin Alan), 1969-
|
| ISBN |
9781908230874 |
|
1908230878 |
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