| Description |
x, 327 pages : illustrations ; 24 cm |
| Series |
Methods in molecular biology ; 11 |
|
Methods in molecular biology (Clifton, N.J.) ; 11
|
| Contents |
Ch. 1. Thiophilic Adsorption Chromatography / T. William Hutchens -- Ch. 2. Immobilized Metal Ion Affinity Chromatography / Tai-Tung Yip and T. William Hutchens -- Ch. 3. Histidine and Affinity Chromatography / Mookambeswaran A. Vijayalakshhmi -- Ch. 4. Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes / James H. Hageman and Glenn D. Kuehn -- Ch. 5. Calcium-Dependent Hydrophobic Interaction Chromatography / Nicholas H. Battey and Michael A. Venis -- Ch. 6. Lectin Affinity Chromatography / Iris West and Owen Goldring -- Ch. 7. Dye-Ligand Affinity Chromatography / Steven J. Burton -- Ch. 8. High-Performance Affinity Chromatography for Protein Separation and Purification / Yannis D. Clonis -- Ch. 9. Immunoaffinity Chromatography / George W. Jack -- Ch. 10. Selection of Antibodies for Immunoaffinity Chromatography / Duncan S. Pepper -- Ch. 11. Some Alternative Coupling Chemistries for Affinity Chromatography / Duncan S. Pepper |
|
Ch. 12. Exploiting Weak Affinities / Sten Ohlson -- Ch. 13. Biospecific Affinity Elution / Robert Kerry Scopes -- Ch. 14. Size-Exclusion High-Performance Liquid Chromatography of Proteins / David A. Harris -- Ch.15. Chromatofocusing / Chee Ming Li and T. William Hutchens -- Ch.16. Ion-Exchange Chromatography of Proteins / Andrew C. Kenney -- Ch.17. Displacement Chromatography of Proteins / Steven M. Cramer -- Ch. 18. Purification of DNA Binding Proteins by Affinity and Ion Exchange Chromatography / David Hornby, Kevin Ford and Paul Shore -- Ch. 19. Making Bacterial Extracts Suitable for Chromatography / Roger F. Sherwood -- Ch. 20. Determination of Purity and Yield / Sudesh B. Mohan |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
| Subject |
Affinity chromatography.
|
|
Proteins -- Analysis.
|
|
Affinity chromatography.
|
|
Chromatographic analysis -- Technique.
|
|
Proteins -- Analysis.
|
| Author |
Fowell, Susan.
|
|
Kenney, Andrew.
|
|
Humana Press.
|
| LC no. |
92003609 |
| ISBN |
0896032132 |
|
