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Title Basic science methods for clinical researchers / edited by Morteza Jalali, Francesca Y.L. Saldanha, Mehdi Jalali
Published London : Academic Press, an imprint of Elsevier, [2017]
©2017

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Description 1 online resource (xxv, 355 pages) : illustrations (chiefly color)
Contents The polymerase chain reaction: PCR, qPCR, and RT-PCR / Mehdi Jalali, Justyna Zaborowska, Morteza Jalali -- Methods of cloning / Alessandro Bertero, Stephanie Brown, Ludovic Vallier -- Whole-mount in situ hybridization and a genotyping method on single Xenopus embryos / Martyna Lukoseviciute, Robert Lea, Shoko Ishibashi, Enrique Amaya -- Microarrays: an introduction and guide to their use / Frederick D. Park, Roman Sasik, Tannishtha Reya -- Analysis of human genetic variations using DNA sequencing / Gregory A. Hawkins -- Western blot / Tomasz Gwozdz, Karel Dorey -- The enzyme-linked immunosorbent assay: the application of ELISA in clinical research / Jefte M. Drijvers, Imad M. Awan, Cory A. Perugino, Ian M. Rosenberg, Shiv Pillai -- Immunofluorescence / Sonali Joshi, Dihua Yu -- Cell culture: growing cells as model systems in vitro / Charis-P. Segeritz, Ludovic Vallier -- Flow cytometry / Eleni Chantzoura, Keisuke Kaji -- Transfection / Semira Sheikh, Amanda S. Coutts, Nicholas B. La Thangue -- In vivo animal modeling: Drosophila / Michael F. Wangler, Hugo J. Bellen -- Zebrafish as a research organism: Danio rerio in biomedical research / John Collin, Paul Martin -- Xenopus as a Model Organism for Biomedical Research / Shoko Ishibashi, Francesca Y.L. Saldanha, Enrique Amaya -- Basic mouse methods for clinician researchers: harnessing the mouse for biomedical research / Laurens J. Lambert, Mandar D. Muzumdar, William M. Rideout III, Tyler Jacks -- Appendices. Legal framework on the scientific use of animals in research / David Pettitt, Adam Pettitt, MacKenna Roberts, James Smith, David Brindley -- Regulatory frameworks for stem cell research / Eleanor Jane Budge, Morteza Jalali -- Using multiple experimental methods to address basic science research questions / Justyna Zaborowska, Morteza Jalali
Summary "Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science"--Publisher's description
Bibliography Includes bibliographical references and index
Notes Online resource; title from electronic title page (ScienceDirect viewed March 30, 2018)
Subject Research.
Medical research personnel.
Science.
Research Design
Science
Research
Research Personnel
research (function)
sciences (philosophy)
science (modern discipline)
SCIENCE -- Study & Teaching.
Science
Medical research personnel
Research
Genre/Form Electronic books
Form Electronic book
Author Jalali, Morteza, editor.
Saldanha, Francesca Y. L., editor.
Jalali, Mehdi, editor.
Davies, S. C. (Sally Claire), 1949- writer of foreword.
LC no. 2016960794
ISBN 9780128030783
012803078X