| Description |
1 online resource (xvii, 537 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1636 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 1636. 1064-3745
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Springer protocols (Series)
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| Contents |
Optogenetic control of Ras/Erk signaling using the Phy-PIF system / Alexander G. Goglia, Maxwell Z. Wilson, Daniel B. DiGiorno, and Jared E. Toettcher -- Dissecting kinase effector signaling using the RapRTAP methodology / Anne-Marie Ray, Jennifer E. Klomp, Kerrie B. Collins, and Andrei V. Karginov -- Single-cell imaging of ERK signaling using fluorescent biosensors / Michael Pargett, Taryn E. Gillies, Carolyn K. Teragawa, Breanne Sparta, and John G. Albeck -- Quantification of cell signaling networks using kinase activity chemosensors / Jon R. Beck, Edward N. Harris, and Cliff I. Stains -- Expression of recombinant phosphoproteins for signal transduction studies / Karl W. Barber and Jesse Rinehart -- Allosteric modulation of Src family kinases with ATP-competitive inhibitors / Ames C. Register, Sujata Chakraborty, and Dustin J. Maly -- Characterization of ligand binding to pseudokinases using a thermal shift assay / Isabelle S. Lucet and James M. Murphy -- Proteomic profiling of protein kinase inhibitor targets by mass spectrometry / Martin Golkowski, Dustin J. Maly, and Shao-En Ong -- Utilizing the luminex magnetic bead-based suspension array for rapid multiplexed phosphoprotein Quantification / Adam Stewart and Udai Banerji -- High-content imaging and RNAi screens for investigating kinase network plasticity / Simon R. Stockwell and Sibylle Mittnacht -- Analysis of drug resistance using kinome-wide functional screens / Katherine R. Singleton, Keith T. Earley, and Lynn E. Heasley -- Identification and validation of driver kinases from next-generation sequencing data / Andri Leonidou, Barrie Peck, and Rachael Natrajan -- Label-free phosphoproteomic approach for kinase signaling analysis / Edmund Wilkes and Pedro R. Cutillas -- Cell-specific labeling for analyzing bidirectional signaling by mass spectrometry / Christopher J. Tape and Claus Jørgensen -- Characterization of the phospho-adhesome by mass Spectrometry-Based Proteomics / Joseph Robertson, Jonathan D. Humphries, Nikki R. Paul, Stacey Warwood, David Knight, Adam Byron, and Martin J. Humphries -- Analysis of phosphotyrosine signaling networks in lung cancer cell lines / Malgorzata Broncel and Paul H. Huang -- Targeted analysis of phosphotyrosine signaling by multiple reaction monitoring mass spectrometry / Leo S. Payne and Paul H. Huang -- Phosphoproteomic analysis of isolated mitochondria in yeast / Margaux Renvoise, Ludovic Bonhomme, Marlene Davanture, Michel Zivy, and Claire Lemaire -- Methodology for comprehensive analysis of toll-like receptor signaling in macrophages / Marijke Koppenol-Raab and Aleksandra Nita-Lazar -- Absolute phosphorylation stoichiometry analysis by motif-targeting quantitative mass spectrometry / Chia-Feng Tsai, Wei-Chi Ku, Yu-Ju Chen, and Yasushi Ishihama -- Identification of plant kinase substrates based on kinase assay-linked phosphoproteomics / Chuan-Chih Hsu, Justine V. Arrington, Liang Xue, and W. Andy Tao -- Mass spectrometry analysis of spatial protein networks by colocalization analysis (COLA) / Faraz K. Mardakheh -- Development of selected reaction monitoring methods to systematically quantify kinase abundance and phosphorylation stoichiometry in human samples / Kirsten Beck, Nathan Camp, Michael Bereman, James Bollinger, Jarrett Egertson, Michael MacCoss, and Alejandro Wolf-Yadlin -- Analysis of signaling networks at the single-cell level using mass cytometry / Shovik Bandyopadhyay, Daniel A.C. Fisher, Olga Malkova, and Stephen T. Oh -- Magnetic resonance spectroscopy (MRS)-based methods for examining cancer metabolism in response to oncogenic kinase drug treatment / Yuen-Li Chung -- Deconstructing the metabolic networks of oncogenic signaling using targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) / George Poulogiannis -- Modeling of receptor tyrosine kinase signaling : computational and experimental protocols / Dirk Fey, Edita Aksamitiene, Anatoly Kiyatkin, and Boris N. Kholodenko -- Interdisciplinary approach for designing kinetic models of the Ras/MAPK signaling pathway / Marcelo S. Reis, Vincent Noel, Matheus H. Dias, Layra L. Albuquerque, Amanda S. Guimaraes, Lulu Wu, Junior Barrera, and Hugo A. Armelin -- Databases and computational tools for evolutionary analysis of protein phosphorylation / Chris Soon Heng Tan -- Informatics approaches for predicting, understanding, and testing cancer drug combinations / Jing Tang -- Target inhibition maps based on responses to kinase inhibitors / Noah Berlow and Ranadip Pal -- Partial least squares regression models for the analysis of kinase signaling / Danielle L. Bourgeois and Pamela K. Kreeger |
| Summary |
This detailed volume describes cutting-edge techniques in three distinct and complementary areas of contemporary kinase biology research. Beginning with a section on synthetic biology, chemical biology, and screening approaches to kinase signaling networks, the book continues with sections on mass spectrometry and metabolic analysis of kinase signaling as well as computational analysis of kinase signaling networks. Written for the highly successful Methods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Kinase Signaling Networks aims to provide scientists with the tools necessary to overcome the technological bottlenecks that limit our ability to gain a deeper mechanistic understanding of kinase biology |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title form PDF title page (SpringerProtocols, viewed July 24, 2017) |
| Subject |
Protein kinases -- Laboratory manuals
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Phosphotransferases.
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Protein kinases.
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Cellular signal transduction.
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Phosphotransferases
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Protein Kinases
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Signal Transduction
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Phosphotransferases
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Cellular signal transduction
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Protein kinases
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Tan, Aik-Choon, editor
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Huang, Paul H., editor
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| ISBN |
9781493971541 |
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1493971549 |
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