| Description |
1 online resource (xi, 180 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1759 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1759.
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| Contents |
880-01 Short Overview of PINK1/Parkin-mediated Mitophagy -- Induction of PINK1/Parkin-Mediated Mitophagy -- Immunocytochemical Monitoring of PINK1/Parkin-mediated Mitophagy in Cultured Cells -- The Use Of Correlative Light-electron Microscopy (CLEM) To Study PINK1/Parkin-mediated Mitophagy -- Observation Of Parkin-mediated Mitophagy in Pancreatic Beta-cells -- Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila -- Assessment of Mitophagy in iPS Cell-derived Neurons -- Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting -- Mitopho8∆60 Assay as a Tool to Quantitatively Measure Mitophagy Activity -- Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants -- Flow Cytometer Monitoring of Bnip3 and Bnip3L/Nix-dependent Mitophagy -- Exploring Micrornas on Nix-Dependent Mitophagy -- Monitoring of Atg5-Independent Mitophagy -- Monitoring of Paternal Mitochondrial Degradation In Caenorhabditis Elegans -- Detection of Hypoxia-induced and Iron Depletion-induced Mitophagy in Mammalian Cells -- Monitoring Mitophagy during Ageing in Caenorhabditis Elegans -- Iron-Starvation Induced Mitophagy in Yeast |
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880-01/(S Short overview / Norihiko Furuya -- Induction of PINK1/parkin-mediated mitophagy / Shigeto Sato and Norihiko Furuya -- Immunocytochemical monitoring of PINK1/parkin-mediated mitophagy in cultured cells / Motoki Fujimaki, Shinji Saiki, Yukiko Sasazawa, Kei-Ichi Ishikawa, Yoko Imamichi, Katsuhiko Sumiyoshi, and Nobutaka Hattori -- Use of vorrelative light-electron microscopy (CLEM) to study PINK1/parkin-mediated mitophagy / Chieko Kishi-Itakura and Folma Buss -- Observation of parkin-mediated mitophagy in pancreatic β-cells / Atsushi Hoshino and Satoaki Matoba -- Monitoring mitochondrial changes by alteration of the PINK1-parkin signaling in Drosophila / Tsuyoshi Inoshita, Kahori Shiba-Fukushima, Hongrui Meng, Nobutaka Hattori, and Yuzuru Imai -- Assessment of mitophagy in iPS cell-derived neurons / Kei-Ichi Ishikawa, Akihiro Yamaguchi, Hideyuki Okano, and Wado Akamatsu -- Investigation of yeast mitophagy with fluorescence microscopy and western blotting / Sachiyo Nagumo and Koji Okamoto -- MitoPho8Δ60 assay as a tool to quantitatively measure mitophagy activity / Zhiyuan Yao, Xu Liu, and Daniel J. Klionsky -- Mitophagy in yeast : a acreen of mitophagy-deficient mutants / Kentaro Furukawa and Tomotake Kanki -- Flow cytometer monitoring of Bnip3- and Bnip3L/nix-dependent mitophagy ./ Matilda Sprung, Ivan Dikic, and Ivana Novak -- Exploring microRNAs on NIX-dependent mitophagy / Wen Li, Hao Chen, Shupeng Li, Guanghong Lin, and Du Feng -- Monitoring of Atg5-independent mitophagy / Satoko Arakawa, Shinya Honda, Satoru Torii, Masatsune Tsujioka, and Shigeomi Shimizu -- Monitoring of paternal mitochondrial degradation in Caenorhabditis elegans / Miyuki Sato and Ken Sato -- Detection of hypoxia-induced and iron depletion-induced mitophagy in mammalian cells / Shun-ichi Yamashita and Tomotake Kanki -- Monitoring mitophagy during aging in Caenorhabditis elegans / Nikolaos Charmpilas, Konstantinos Kounakis, and Nektarios Tavernarakis -- Monitoring of iron depletion-induced mitophagy in pathogenic yeast / Koichi Tanabe and Minoru Nagi -- Dopaminergic neuron-specific autophagy-deficient mice / Shigeto Sato and Nobutaka Hattori |
| Summary |
This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson's disease, especially young onset Parkinson's disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLinks, viewed August 20, 2018) |
| Subject |
Mitochondria -- Laboratory manuals
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Lysosomes -- Laboratory manuals
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Mitophagy
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Cellular biology (cytology)
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Lysosomes
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Mitochondria
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Hattori, Nobutaka, editor
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Saiki, Shinji, editor
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| LC no. |
2018947388 |
| ISBN |
9781493977505 |
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1493977504 |
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