| Description |
1 online resource (310 pages) |
| Contents |
880-01 Front Cover; New and Future Developments in Microbial Biotechnology and Bioengineering; Copyright Page; Contents; List of Contributors; I. Penicillium: Biology to Biotechnology; 1 Biodiversity of the Genus Penicillium in Different Habitats; 1.1 Introduction; 1.2 Isolation and Characterization of Penicillium; 1.3 Molecular Diversity and Phylogenetic Analysis; 1.4 Distribution and Abundance of Penicillium; 1.4.1 Low/High-Temperature Habitat; 1.4.2 Saline Habitat; 1.4.3 Acidic/Alkaline Habitat; 1.4.4 Penicillium Associated With Plants and Fruits; 1.5 Conclusion and Future Prospects; References |
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880-01 2.3.2.5.1 PCR Detection of Ochratoxin A-Producing Penicillium2.3.2.5.2 PCR Detection of Patulin-Producing Penicillium; 2.4 Morphological and Molecular Identification of Penicillium species; 2.4.1 Morphological Characters; 2.4.1.1 Culture Conditions; 2.4.1.2 Macromorphology; 2.4.1.3 Micromorphology; 2.4.2 Phylogenetic Analysis of Penicillium; 2.4.3 Molecular Methods for the Characterization of Penicillium spp.; 2.4.3.1 Sequence Markers; 2.4.3.1.1 β-Tubulin as a Secondary Marker in Practice; 2.4.3.1.2 Extrolite Data |
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2 Understanding the Diversity of Penicillium Using Next-Generation Sequencing2.1 Introduction; 2.2 Isolation of Penicillium species; 2.2.1 Isolation From Air; 2.2.2 Isolation From Soil Samples; 2.2.3 Isolation From Food Samples; 2.3 Detection of Penicillium species; 2.3.1 Conventional Techniques; 2.3.2 Molecular Techniques; 2.3.2.1 Preparation of Fungal Culture for Toxin Detection; 2.3.2.2 DNA Extraction From Fungal Cultures for Molecular Identification; 2.3.2.3 Determining DNA Concentration and Purity; 2.3.2.4 Polymerase Chain Reaction; 2.3.2.5 PCR Detection of Penicillium Species |
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2.4.3.1.3 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Fingerprinting (MALDI-TOF MS)2.4.3.2 Cloning and Sequencing of PCR Products; 2.4.3.2.1 Preparation and Analysis of DNA Heteroduplexes; 2.4.3.2.2 Identification of Polymorphic Genetic Loci; 2.4.3.3 Next-Generation Sequencing; 2.4.3.3.1 Roche 454 FLX Pyrosequencer; 2.4.3.3.2 Illumina Genome Analyzer; 2.4.3.3.3 Applied Biosystem SOLiD Sequencer; 2.4.3.3.4 Heliscope Single-Molecule Sequencer; 2.4.3.3.5 Ion Personal Genome Machine (PGM); 2.4.3.3.6 RNA-Seq |
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2.4.3.3.7 Pacific Biosciences SMRT Sequencing DNA Sequencer2.4.3.4 Submitting DNA Fragment for Sequencing; 2.4.3.4.1 Using Bioinformatics; 2.5 Conclusions and Future Perspectives; References; Further Reading; II. Secondary Metabolism; 3 Secondary Metabolism and Antimicrobial Metabolites of Penicillium; 3.1 Introduction; 3.2 Secondary Metabolism in Fungi; 3.2.1 Secondary Metabolism as Differentiation; 3.3 Microorganisms as Sources of Secondary Metabolites; 3.3.1 Bioactivities of Microbial Secondary Metabolites; 3.3.2 Biotechnological Importance of Fungi; 3.3.2.1 Ergot Alkaloids |
| Notes |
3.3.2.2 Diketopiperazines |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (EBSCO, viewed September 28, 2017) |
| Subject |
Microbial biotechnology.
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| Form |
Electronic book
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| Author |
Gupta, Vijai G., editor
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Rodriguez-Couto, Susana, editor
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| ISBN |
0444635122 (electronic bk.) |
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9780444635129 (electronic bk.) |
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9780444635013 |
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