Title Cancer genomics and proteomics : methods and protocols / edited by Paul B. Fisher

Published Totowa, N.J. : Humana Press, ©2007

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Description 1 online resource (xvi, 358 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; 383 Methods in molecular biology (Clifton, N.J.) ; v. 383 Contents Reciprocal subtraction differential RNA display (RSDD) : an efficient technology for cloning differentially expressed genes / Devanand Sarkar, Dong-chul Kang, and Paul B. Fisher -- Cloning differentially expressed genes using rapid subtraction hybridization (RaSH) / Habib Boukerche [and others] -- The application of differential display as a gene profiling tool / Ken Chien-Neng Chang [and others] -- Serial analysis of gene expression (SAGE) / Fred van Ruissen and Frank Baas -- Gene expression profile analysis of tumors / Katia Basso and Riccardo Dalla-Favera -- cDNA microarray and bioinformatic analysis of nuclear factor [kappa]B related genes in squamous cell carcinoma / Zhong Chen [and others] -- Gene profiling uncovers retinoid target genes / Yan Ma [and others] -- Complete open reading frame (C-ORF) technique : rapid and efficient method for obtaining complete protein coding sequences / Dong-chul Kang and Paul B. Fisher -- Chromatin immunoprecipitation assays : molecular analysis of chromatin modification and gene regulation / Piyali Dasgupta and Srikumar P. Chellappan -- Manipulating genes and gene copy number by bacterial artificial chromosome transfection / Stephanie F. Phelps, Sharon Illenye, and Nicholas H. Heintz -- Molecular cytogenetic applications in analysis of the cancer genome / Pulivarthi H. Rao, Subhadra V. Nandula, and Vundavalli V. Murty -- Monitoring methylation and gene expression in cancer / Hetty Carraway and James Herman -- The use of phage display peptide libraries for basic and translational research / Renee Brissette and Neil I. Goldstein -- Yeast and mammalian two-hybrid systems for studying protein-protein interactions / Shu-ichi Matsuzawa and John C. Reed -- Ribonomic and short hairpin RNA gene silencing methods to explore functional gene programs associated with tumor growth arrest / Timothy E. Baroni [and others] -- Surface-epitope masking (SEM) : an immunological subtraction approach for developing monoclonal antibodies targeting surface-expressed molecules / Neil I. Goldstein and Paul B. Fisher -- Approaches for monitoring signal transduction changes in normal and cancer cells / Paul Dent [and others] -- PKR in innate immunity, cancer, and viral oncolysis / Siddharth Balachandran and Glen N. Barber -- Analysis of transformation and tumorigenicity using mouse embryonic fibroblast cells / Hong Sun and Reshma Taneja -- Analysis of growth properties and cell cycle regulation using mouse embryonic fibroblast cells / Hong Sun, Neriman Tuba Gulbagci, and Reshma Taneja -- Reverse phase protein microarrays for monitoring biological responses / Virginia Espina [and others] -- Protein crystallization / Champion Deivanayagam, William J. Cook, and Mark R. Walter Summary Cancer Genomics and Proteomics: Methods and Protocols provides a compendium of techniques and applications that will be of profound use to researchers interested in gene identification and function. The approaches described in this volume are state-of-the-art and may be tailored to ongoing individual or planned research projects. Reviews are written by experts in specific aspects of gene identification and full-length gene cloning, gene profiling (microarrays), chromatin modification of gene regulation, bacterial artificial chromosomes, cancer cytogenetic analyses, and gene methylation. Chapters also discuss topics such as phage display, yeast and mammalian two-hybrid systems, RNA silencing, monoclonal antibody production, kinases and signal transduction, PKR, analyses of mouse embryo fibroblasts, protein microarrays, and protein crystallization. Cancer Genomics and Proteomics: Methods and Protocols will be of interest to molecular biologists, geneticists, cell biologists, and biochemists involved in studying genes associated with and regulating important physiological processes. This volume will serve as a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena Analysis neoplasms humane ziekten human diseases geneeskunde medicine genen genes moleculaire biologie molecular biology biologische technieken biological techniques eiwitexpressieanalyse proteomics genexpressieanalyse genomics protocollen protocols gene discovery genisolatie gene isolation functionele genomica functional genomics Bioinformatics (General) Medicine (General) Bioinformatica (algemeen) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject Cancer -- Genetic aspects. Cancer -- Molecular aspects. Genomics. Proteomics. Genetics -- Technique. Neoplasms -- genetics Genetic Techniques Genomics -- methods Proteomics -- methods Genomics Proteomics MEDICAL -- Oncology. HEALTH & FITNESS -- Diseases -- Cancer. Cancer -- Genetic aspects. Cancer -- Molecular aspects. Genomics. Proteomics. Genetics -- Technique Cancer -- Genetic aspects Cancer -- Molecular aspects Genomics Proteomics Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Fisher, Paul B. ISBN 1597453358 9781597453356 1280971649 9781280971648 9786610971640 6610971641

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