Title Protocols for neural cell culture / edited by Sergey Fedoroff and Arleen Richardson

Edition 3rd ed Published Totowa, N.J. : Humana Press, ©2001

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Description 1 online resource (xxii, 362 pages) : illustrations Series Springer Protocols Handbooks Springer protocols (Series) Contents In vitro assays for axonal growth and targeting / Valérie Castellani and Jürgen Bolz -- Interface organotypic hippocampal slice cultures / Dominique Muller [and others] -- Slice cultures for study of microglia / Carol A. Colton, Meggan Czapiga, and Toby N. Behar -- Microexplant cultures of the cerebellum / Bernard Rogister and Gustave Moonen -- Construction and use of compartmented cultures for studies of cell biology of neurons / Robert B. Campenot and Grace Martin -- Chick spinal somatic motoneurons in culture / Bernhard H.J. Juurlink -- Primary cultures of sympathetic ganglia / Mary I. Johnson -- Primary cell cultures for the study of myelination / Mary I. Johnson, Richard P. Bunge, and Patrick M. Wood -- Preparation of astrocyte, oligodendrocyte, and microglia cultures for primary rat cerebral cultures / Ruth Cole and Jean de Vellis -- Culture of glial cells from human brain biopsies / V. Wee Yong and Jack P. Antel Cultures of astroglia and microglia from primary cultures of mouse neopallium / Sergey Fedoroff and Arleen Richardson -- Cultures of oligodendroblasts from primary cultures of rat neopallium / Bernhard H.J. Juurlink, Shawn K. Thorburne, and Richard M. Devon -- Olfactory ensheathing cell cultures / Ronald Doucette -- Cultures of stem cells of the central nervous system / Angela Gritti, Rossella Galli, and Angelo Luigi Vescovi -- Aggregating neural cell cultures / Paul Honegger and Florianne Monnet-Tschudi -- Neural cell lines / Kaoru Murayama [and others] -- Virus vectors for gene therapy of the nervous system / Sonia N. Yeung and Frank Tufaro -- Preparation of substrata for in vitro culture of neurons / Paul C. Letourneau -- Serum-fee media for neural cell cultures: adult and embryonic / Paul J. Price and Gregory J. Brewer Development of multiple-well plate biological assays / John w. Harbell -- Neural cell counting: cell number determination in microplate cultures / Marston Manthorpe -- Assays for neuronal cell migration / Toby N. Behar -- Colony culture: plating efficiency assay and cloning / Sergey Fedoroff and Arleen Richardson -- Hybridoma cultures for production of antibodies: purification and specificity of antibodies / J. Denry Sato -- Elimination of cell types from mixed neural cell cultures / Richard M. Devon -- Quantification of cells in culture / Arleen Richardson and Sergey Fedoroff -- Tips for tissue culture / Arleen Richardson and Sergey Fedoroff Summary In Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures. Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Nerve tissue -- Cultures and culture media -- Laboratory manuals Neurons -- Growth -- Laboratory manuals Nerve tissue -- Cultures and culture media. Neurons -- Growth. Nerve Tissue -- cytology Tissue Culture -- methods Neurons -- cytology Culture Techniques -- methods MEDICAL -- Histology. Nerve tissue -- Cultures and culture media Neurons -- Growth Neuronen. Celkweek. Laboratoriumonderzoek. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Fedoroff, Sergey. Richardson, Arleen. LC no. 00059763 ISBN 9781592592074 1592592074 0585403171 9780585403175

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