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Author Eckford, Paul D. W

Title Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (599 secs.)
Series Biology
Summary Described here is a rapid and effective procedure for functional reconstitution of purified wild-type and mutant CFTR protein that preserves activity for this chloride channel, which is defective in Cystic Fibrosis. Iodide efflux from reconstituted proteoliposomes mediated by CFTR allows studies of channel activity and the effects of small molecules
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Biology
Form Streaming audio