| Description |
1 online resource (xi, 221 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1790 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1790. 1064-3745
|
| Contents |
Dual modality imaging of promoter activity as a surrogate for gene expression and function / Ajit Dhadve, Bhushan Thakur, and Pritha Ray -- Construction of dual modality optical reporter gene constructs for bioluminescent and fluorescent imaging / Ajit Dhadve, Bhushan Thakur, and Pritha Ray -- Optical bioluminescence protocol for imaging mice / David Stout and John David -- Dual reporter bioluminescence imaging with NanoLuc and firefly luciferase / Anne E. Gibbons, Kathryn E. Luker, and Gary D. Luker -- Reporter-based bret sensors for measuring biological functions in vivo / Maitreyi Rathod, Arijit Mal, and Abhijit De -- Fluorescence imaging of mycobacterial infection in live mice using fluorescent protein-expressing strains / Ying Kong and Jeffrey D. Cirillo -- In vivo bacterial imaging using bioluminescence / Mariette Barbier, Justin Bevere, and F. Heath Damron -- Production of GFP and luciferase-expressing reporter macrophages for in vivo bioluminescence imaging / Jukka Pajarinen, Tzu-Hua Lin, and Stuart B. Goodman -- Hypoxia-induced reporter genes with different half-lives / Balaji Krishnamachary, Pierre Danhier, Samata Kakkad, Santosh K. Bharti, and Zaver M. Bhujwalla -- Visualization of immune cell reconstitution by bioluminescent imaging / Purnima Dubey -- Thymidine kinase PET reporter gene imaging of cancer cells in vivo / Melissa M. McCracken -- Ex vivo radiolabeling and in vivo PET imaging of T cells expressing nuclear reporter genes / Maxim A. Moroz, Pat Zanzonico, Jason T. Lee, and Vladimir Ponomarev -- Imaging T cell dynamics and function using PET and human nuclear reporter genes / Jason T. Lee, Maxim A. Moroz, and Vladimir Ponomarev -- Positron emission tomography imaging of tumor apoptosis with a caspase-sensitive nano-aggregation tracer [18F]C-SNAT / Zixin Chen and Jianghong Rao -- Practical guidelines for Cerenkov luminescence imaging with clinically relevant isotopes / Nikunj B. Bhatt, Darpan N. Pandya, William A. Dezarn, Frank C. Marini, Dawen Zhao, William H. Gmeiner, Pierre L. Triozzi, and Thaddeus J. Wadas -- Synthesis, prification, characterization, and imaging of Cy3-functionalized fluorescent silver nanoparticles in 2D and 3D tumor models / Jessica Swanner and Ravi Singh |
| Summary |
This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed June 4, 2018) |
| Subject |
Reporter genes -- Laboratory manuals
|
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Molecular genetics -- Laboratory manuals
|
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Reporter genes.
|
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Genes, Reporter
|
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Luminescent Measurements -- methods
|
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Optical Imaging -- methods
|
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Radiology.
|
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Medical -- Biochemistry.
|
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Molecular genetics
|
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Reporter genes
|
| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Dubey, Purnima, editor
|
| ISBN |
9781493978601 |
|
1493978608 |
|
