| Description |
1 online resource (xi, 334 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 636 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 636. 1064-3745
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Springer protocols (Series)
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| Contents |
Human embryonic stem cell derivation, maintenance, and differentiation to trophoblast / Ge Lin, Kristen Martins-Taylor, and Ren-He Xu -- Isolation and maintenance of mouse epiblast stem cells / Josh G. Chenoweth and Paul J. Tesar -- Functional assays for hematopoietic stem cell self-renewal / John M. Perry and Linheng Li -- Isolation procedure and characterization of multipotent adult progenitor cells from rat bone marrow / Kartik Subramanian [and others] -- Generation of functional insulin-producing cells from human embryonic stem cells in vitro / Yan Shi -- Mesoderm cell development from ES cells / Takumi Era -- Directed differentiation of red blood cells from human embryonic stem cells / Shi-Jiang Lu [and others] -- Directed differentiation of neural-stem cells and subtype-specific neurons from hESCs / Bao-Yang Hu and Su-Chun Zhang -- Directing human embryonic stem cells to a retinal fate / Thomas A. Reh, Deepak Lamba, and Juliane Gust -- Bovine somatic cell nuclear transfer / Pablo J. Ross and Jose B. Cibelli -- Cell fusion-induced reprogramming / Jeong Tae Do and Hans R. Schöler -- An improved method for generating and identifying human induced pluripotent stem cells / Prashant Mali [and others] -- Using small molecules to improve generation of induced pluripotent stem cells from somatic cells / Caroline Desponts and Sheng Ding -- Reprogramming of committed lymphoid cells by enforced transcription factor expression / Huafeng Xie, Catherine V. Laiosa, and Thomas Graf -- Reprogramming of B cells / César Cobaleda -- Adult cell fate reprogramming: converting liver to pancreas / Irit Meivar-Levy and Sarah Ferber -- In vitro reprogramming of pancreatic cells to hepatocytes / Daniel Eberhard [and others] -- Generation of novel rat and human pluripotent stem cells by reprogramming and chemical approaches / Wenlin Li and Sheng Ding -- Small molecule screen in zebrafish and HSC expansion / Eirini Trompouki and Leonard I. Zon -- Zebrafish small molecule screen in reprogramming/cell fate modulation / Jing-Ruey J. Yeh and Kathleen M. Munson |
| Summary |
Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls. Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer |
| Analysis |
celbiologie |
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cellular biology |
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celkweek |
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cell culture |
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stamcellen |
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stem cells |
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Cellular Biology |
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Celbiologie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Cellular therapy.
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Cell transplantation.
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Stem cells.
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Cellular Reprogramming
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Stem Cells
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Cell- and Tissue-Based Therapy
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Stem cells
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Cell transplantation
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Cellular therapy
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Ding, Sheng, 1975-
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| ISBN |
9781607616917 |
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1607616912 |
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