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Author Dhungel, Pragyesh

Title In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (538 secs.)
Series Immunology and Infection
Summary We present a protocol to study mRNA translation regulation in poxvirus-infected cells using in vitro Transcribed RNA-based luciferase reporter assay. The assay can be used for studying translation regulation by cis-elements of an mRNA, including 5'-untranslated region (UTR) and 3'-UTR. Different translation initiation modes can also be examined using this method
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Immunology and Infection
Form Streaming audio