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Title Auxins and cytokinins in plant biology : methods and protocols / edited by Thomas Dandekar, Muhammad Naseem
Published New York : Humana Press : Springer, [2017]
©2017

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Description 1 online resource (x, 204 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 1569
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 1569. 1064-3745
Springer protocols (Series)
Contents Methodological advances in auxin and cytokinin biology / Andrej Hurný and Eva Benková -- Analytical determination of auxins and cytokinins / Petre I. Dobrev, Klára Hoyerová, and Jan Petrášek -- Manipulation of auxin and cytokinin balance during the Plasmodiophora brassicae-Arabidopsis thaliana interaction / Jutta Ludwig-Müller, Susann Auer, Sabine Jülke, and Sabine Marschollek -- Microbial manipulation of auxins and cytokinins in plants / Anwar Hussain, Ihsan Ullah, and Shahida Hasnain -- Standardized method to assess infection rates of root-knot and cyst nematodes in Arabidopsis thaliana mutants with alterations in root development related to auxin and cytokinin signaling / Rocío Olmo, Ana Cláudia Silva, Fernando E. Díaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar-- Reconstruction of an immune dynamic model to simulate the contrasting role of auxin and cytokinin in plant immunity / Martin Kaltdorf, Thomas Dandekar, and Muhammad Naseem -- Interplay between auxin and cytokinin and its impact on mitogen activated protein kinase (MAPK) / Pallavi Singh and Alok Krishna Sinha -- Quantification of cytokinin levels and responses in abiotic stresses / Alfonso Albacete -- Assessment of cytokinin-induced immunity through quantification of Hyaloperonospora arabidopsidis infection in Arabidopsis thaliana / Ruth A. Watson and Cristiana T. Argueso -- Real-time genetic manipulations of the cytokinin pathway : a tool for laboratory and field studies / Martin Schäfer and Stefan Meldau -- Modulating the levels of plant hormone cytokinins at the host-pathogen interface / Muhammad Naseem, Shabana Shams, and Thomas Roitsch -- Analyzing cytokinin responses during plant-nematode interactions / Florian M.W. Grundler and Shahid Siddique -- Examining H2O2 Production in Arabidopsis leaves upon challenge by cytokinin / Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed -- Systems biology methodology combining transcriptome and interactome datasets to assess the implications of cytokinin signaling for plant immune networks / Meik Kunz, Thomas Dandekar, and Muhammad Naseem -- Monitoring of crosstalk between jasmonate and auxin in the framework of plant stress responses of roots / Víctor Carrasco Loba, Marta-Marina Pérez Alonso, and Stephan Pollmann -- High-throughput protoplast trans-activation (PTA) screening to define transcription factors in auxin-mediated gene regulation / Nora Wehner, Jörn Herfert, Wolfgang Dröge-Laser, and Christoph Weiste
Summary This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins
Bibliography Includes bibliographical references and index
Notes Online resource; title form PDF title page (SpringerProtocols, viewed March 8, 2017)
Subject Auxin.
Cytokinins.
Plants.
Botany.
Indoleacetic acid.
Plants
Plant Cells -- pathology
Plant Cells -- immunology
Indoleacetic Acids
Cytokinins
vegetation.
Botany & plant sciences.
Science -- Life Sciences -- Botany.
Plants
Indoleacetic acid
Botany
Auxin
Cytokinins
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Dandekar, Thomas, 1960- editor.
Naseem, Muhammad, editor
ISBN 9781493968312
1493968319
1493968297
9781493968299