| Description |
1 online resource (690 p.) |
| Contents |
880-01 Intro -- Preface -- Contents -- Abbreviations -- Part I: Pharmaceutical Biotechnology: The Science, Techniques, and Important Concepts -- 1: Molecular Biotechnology: From DNA Sequence to Therapeutic Protein -- Introduction -- Pharmaceutical Biotechnology, Why This Book, Why This Chapter? -- From an In Silico DNA Sequence to a Therapeutic Protein -- Synthetic DNA Production -- Alternative Sources for Cloned DNA Sequences -- Cloning DNA Sequences into an Expression Vector -- Monoclonal Antibodies -- Yields -- Conclusion -- Self-Assessment Questions -- Recommended reading and references |
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880-01/(S 2: Biophysical and Biochemical Characteristics of Therapeutic Proteins -- Introduction -- Protein Structure -- α-Helix -- β-Sheet -- Loops and Turns -- Hydrophobic Interactions -- Hydrogen Bonds -- Electrostatic Interactions -- Van der Waals Interactions -- Protein Folding -- Self-Assessment Questions -- Further Reading -- 3: Stability and Characterization of Protein- and Nucleotide-Based Therapeutics -- Introduction -- Analytical Toolbox: General Overview -- Chromatography -- Electrophoresis -- Mass Spectrometry -- Spectroscopic and Other Techniques for Studying Higher Order Structure |
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Protein Stability: What Can Go Wrong and How to Measure It? -- Protein Modifications Introducing Changes in Charge Heterogeneity -- Deamidation and Isomerization -- Pyro-Glu Formation -- Glycation -- Additional Modifications Inducing Changes in Protein Charge Heterogeneity -- Measuring Changes in Protein Charge Heterogeneity -- Ion-Exchange Chromatography (IEC) -- Isoelectric Focusing (IEF/cIEF) -- Capillary Zone Elecrophoresis (CZE) -- Protein Modifications Introducing Changes in Size -- Protein Fragmentation -- Protein Aggregation -- Measuring Changes in Protein Size |
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Size-Exclusion Chromatography -- Polyacrylamide Gel Electrophoresis ((SDS)-PAGE) -- Detection of Proteins Within Polyacrylamide Gels -- Blotting Techniques -- Capillary Electrophoresis Sodium Dodecyl Sulfate (CE-SDS) -- Asymmetric Field Flow Field Fractionation -- Techniques for Measuring Sub-visible Particles -- Protein Modifications Introducing Changes in Hydrophobicity -- Protein Oxidation -- Measuring Changes in Protein Hydrophobicity -- Reversed-Phase High-Performance Liquid Chromatography -- Hydrophobic Interaction Chromatography -- Two-Dimensional (Hyphenated) Techniques |
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2-Dimensional Gel Electrophoresis and Differential Gel Electrophoresis -- 2-Dimensional Chromatography -- Modifications to the Higher Order Structure of Proteins -- Measuring Changes in Higher Order Structure of Proteins -- Lower Resolution Techniques: CD, FTIR, Fluorescence -- Higher Resolution Techniques -- Stability and Characterization of Nucleotide-Based Pharmaceuticals -- Chemical Stability of Nucleotide-Based Pharmaceuticals -- Hydrolytic Degradation -- Oxidation -- Measuring Chemical Modifications of Nucleotide-Based Pharmaceuticals |
| Summary |
This introductory text explains both the basic science, production, quality, dosage forms, administration, economic and regulatory aspects and the clinical applications of biotechnology-derived pharmaceuticals. It serves as a complete one-stop source for undergraduate/graduate pharmacists and pharmaceutical science students. An additional important audience are pharmaceutical scientists in industry and academia, particularly those who have not received formal training in pharmaceutical biotechnology and are inexperienced in this field. The rapid growth and advances in the field made it necessary to revise this textbook in order to continue providing up-to-date information and introduce readers to cutting edge knowledge and technology of this field. This Sixth Edition completely updates the previous edition and includes additional coverage on new approaches such as oligonucleotides, siRNA, mRNA, gene therapy, cell therapies, monoclonal antibodies and vaccines. With more than 3-million-chapter downloads, the fifth edition of the textbook has achieved widespread distribution as a key educational resource for the field of pharmaceutical biotechnology |
| Notes |
Higher Order Structure Modifications in Nucleotide-Based Pharmaceuticals |
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Online resource; title from PDF title page (SpringerLink, viewed February 14, 2024) |
| Subject |
Pharmaceutical biotechnology.
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Pharmaceutical technology.
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Technology, Pharmaceutical
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| Form |
Electronic book
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| Author |
Crommelin, D. J. A. (Daan J. A.)
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Sindelar, Robert D
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Meibohm, Bernd
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| ISBN |
9783031300233 |
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3031300238 |
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