| Description |
1 online resource (xii, 289 pages) : illustrations |
| Series |
Methods in molecular biology ; 260 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 260
|
| Contents |
Mobile genetic elements as natural tools for genome evolution / Wolfgang J. Miller and Pierre Capy -- Detection of transposable elements in drosophila salivary gland polytene chromosomes by in situ hybridazation / Christian Biémont, Laurence Monti-Dedieu, and Françoise Lemeunier -- Fluorescent in situ hybridazation with transposable element probes to mitotic chromosomal heterochromatin of drosophila / Patrizio Dimitri -- Computational analysis of individual drosophila flies / Nikolai Junakovic -- Computational analysis of transposable element sequences / I. King Jordan and Nathan J. Bowen -- Biochemical analysis of long terminal repeat retrotransposons / Andreas Bachmair [and others] -- Tn5 as a molecular genetics tool : in vitro transposition and the coupling of in vitro technologies with in vivo transposition / William S. Reznikoff, Igor Y. Goryshin, and Jerry J. Jendrisak -- Gene trageting in drosophila / Gregory B. Gloor -- TE-based mutagenesis systems in plants : a gene family approach / Michiel Vandenbussche and Tom Gerats -- The use of double-stranded RNA to knock down specific gene activity / Mary K. Montgomery -- The application of LTR retrotransposons as molecular marker in plants / Alan H. Schulman, Andrew J. Flavell, and T.H. Noel Ellis -- MITE display / Alexandra M. Casa, Alexander Nagel, and Susan R. Wessler -- Retroposon mapping in molecular systematics / Norihiro Okada, Andrew M. Shedlock, and Masato Niakaido -- Transformation systems in insects / David A. O'Brochta and Peter W. Atkinson -- Transposable elements for transgenesis and insertional mutagenesis in vertebrates : a contemporary review of experimental strategies / Zoltán Ivics and Zsuzsanna Izsvák |
| Summary |
Transposable elements (TEs)-DNA sequences that are capable of moving from one chromosome location to another-are found in all living organisms. They have been increasingly investigated in a wide spectrum of species, including bacteria, plants, fungi, and animals. In Mobile Genetic Elements: Protocols and Genomic Applications, leading experts describe in step-by-step detail their most productive transposon-based methods and strategies for studying genome structure, function, and evolution. These readily reproducible techniques cover a broad range, including mutagenesis, transgenesis, gene silencing, and molecular systematics. Among the highlights are a series of DNA hybridization methods for analyzing the distribution and dynamics of mobile DNA at the hosts' genomic level, techniques for studying LTR retrotransposons in heterologous host systems, and mutagenesis protocols for investigating gene functions in a broad range of organisms. Additional methods deal with highly informative sets of polymorphic markers, RNAi technology in gene silencing, and applications during transgenesis. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Mobile Genetic Elements: Protocols and Genomic Applications offers investigators powerful genetic tools for dissecting the function of a specific gene, elaborating on the mechanisms leading to genetic change and diversity, and studying the evolutionary impact of mobile DNA on the biology and evolution of organisms |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Mobile genetic elements -- Laboratory manuals
|
|
Interspersed Repetitive Sequences
|
|
Genomics -- methods
|
|
Molecular Biology -- methods
|
|
Sequence Analysis -- methods
|
|
SCIENCE -- Life Sciences -- Genetics & Genomics.
|
|
Mobile genetic elements
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Miller, Wolfgang J
|
|
Capy, Pierre
|
| LC no. |
2003018232 |
| ISBN |
1592597556 |
|
9781592597550 |
|
1588290077 |
|
9781588290076 |
|
