| Description |
1 online resource (x, 206 pages) : illustrations |
| Series |
Springer protocols |
|
Methods in molecular biology, 1064-3745 ; 499 |
|
Springer protocols (Series)
|
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Methods in molecular biology (Clifton, N.J.) ; v. 499
|
| Contents |
Isolation of dendritic cells from human blood for in vitro interaction studies with fungal antigens / T. Sreevalsan -- Detection and quantitation of antifungal SIgA antibodies in body fluids / M.F. Cole -- Phagocytosis and killing assays for Candida species / C. Du and R.A. Calderone -- Immunization protocols for use in animal models of candidiasis / E. Segal and H. Sandovsky-Losica -- Penetration of antifungal agents through Candida biofilms / L.J. Douglas -- Candida biofilm analysis in the artificial throat using FISH / B.P. Krom [and others] -- Conditions for optimal Candida biofilm development in microtiter plates / B.P. Krom [and others] -- Animal models of candidiasis / C.J. Clancy, S. Cheng and M.H. Nguyen -- Candida albicans gene expression in an in vivo infection model / M.D. Kruppa -- In vitro and ex vivo assays of virulence in Candida albicans / R.A. Calderone -- Biotyping of Candida albicans and other fungi by yeast killer toxins sensitivity / L. Polonelli and S. Conti -- DNA fingerprinting Candida species / C. Pujol and D.R. Soll -- Application of tandem-affinity purification to Candida albicans / C. Blackwell and J.D. Brown -- Preparation of samples for proteomic analysis of the Candida albicans cell wall / N. Chauhan -- Reporter gene assays in Candida albicans / J. Sturtevant -- Genetic transformation of Candida albicans / A.M. Ramon and W.A. Fonzi -- Large-scale gene disruption using the UAU1 cassette / C.J. Nobile and A.P. Mitchell -- Standard growth media and common techniques for use with Candida albicans / N. Chauhan and M.D. Kruppa |
| Summary |
Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiaisis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology |
| Analysis |
candida albicans |
|
candidose |
|
candidosis |
|
mycosen |
|
mycoses |
|
laboratoriummethoden |
|
laboratory methods |
|
moleculaire biologie |
|
molecular biology |
|
protocollen |
|
protocols |
|
Medicine (General) |
|
Biological Techniques |
|
Geneeskunde (algemeen) |
|
Biologische technieken |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Candida albicans.
|
|
Candidiasis.
|
|
Pathogenic fungi.
|
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Candida albicans -- pathogenicity
|
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Candida albicans -- genetics
|
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Candida albicans
|
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Candidiasis
|
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Candida albicans
|
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Candidiasis
|
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Pathogenic fungi
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Cihlar, Ronald L.
|
|
Calderone, Richard A., 1942-
|
| ISBN |
9781603271516 |
|
1603271511 |
|
