| Description |
1 online resource (liii, 851 pages) : illustrations |
| Series |
Methods in enzymology, 0076-6879 ; v. 463 |
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Methods in enzymology ; v. 463
|
| Contents |
Why purify enzymes? / A. Kornberg -- Strategies and considerations for protein purifications / S. Linn -- Use of bioinformatics in planning a protein purification / R.R. Burgess -- Preparing a purification summary table / R.R. Burgess -- Setting up a laboratory / M.P. Deutscher -- Buffers: principles and practice / V.S. Stoll and J.S. Blanchard -- Measurement of enzyme activity / T.K. Harris and M.M. Keshwani -- Quantitation of protein / J.E. Noble and M.J. Bailey -- Concentration of proteins and removal of solutes / D.R. Evans, J.K. Romero and M. Westoby -- Maintaining protein stability / M.P. Deutscher -- Selecting an appropriate method for expressing a recombinant protein / W.H. Brondyk -- Bacterial systems for production of heterologous proteins / S. Zerbs, A.M. Frank and F.R. Collart -- Expression in the yeast Pichia pastoris / J.M. Cregg ... [et al.] -- Baculovirus-insect cell expression systems / D.L. Jarvis -- Recombinant protein production by transient gene transfer into Mammalian cells / S. Geisse and C. Fux -- Tagging for protein expression / A. Malhotra -- Refolding solubilized inclusion body proteins / R.R. Burgess -- Advances in preparation of biological extracts for protein purification / A.C. Grabski -- Isolation of subcellular organelles and structures / U. Michelsen and J. von Hagen -- Protein precipitation techniques / R.R. Burgess -- Affi-Gel Blue for nucleic acid removal and early enrichment of nucleotide binding proteins / M.P. Deutscher -- Ion-exchange chromatography / A. Jungbauer and R. Hahn -- Gel filtration / E. Stellwagen -- Protein chromatography on hydroxyapatite columns / L.J. Cummings, M.A. Snyder and K. Brisack -- Theory and use of hydrophobic interaction chromatography in protein purification applications / J.T. McCue -- Affinity chromatography: general methods / M. Urh, D. Simpson and K. Zhao -- Immobilized-metal affinity chromatography (IMAC): a review / H. Block ... [et al.] -- Identification, production, and use of polyol-responsive monoclonal antibodies for immunoaffinity chromatography / N.E. Thompson ... [et al.] -- One-dimensional gel electrophoresis / D.E. Garfin -- Isoelectric focusing and two-dimensional gel electrophoresis / D.B. Friedman, S. Hoving and R. Westermeier -- Protein gel staining methods: an introduction and overview / T.H. Steinberg -- Elution of proteins from gels / R.R. Burgess -- Performing and optimizing Western blots with an emphasis on chemiluminescent detection / A. Alegria-Schaffer, A. Lodge and K. Vattem -- Detergents: an overview / D. Linke -- Purification of membrane proteins / S.H. Lin and G. Guidotti -- Purification of recombinant G-protein-coupled receptors / R. Grisshammer -- Cell-free translation of integral membrane proteins into unilamelar liposomes / M.A. Goren ... [et al.] -- Determination of protein purity / D.G. Rhodes and T.M. Laue -- Determination of size, molecular weight, and presence of subunits / D.G. Rhodes, R.E. Bossio and T.M. Laue -- Identification and quantification of protein posttranslational modifications / A.R. Farley and A.J. Link -- Parallel methods for expression and purification / S.A. Lesley -- Techniques to isolate O2-sensitive proteins: [4Fe-4S]-FNR as an example / A. Yan and P.J. Kiley -- Rethinking your purification procedure / M.P. Deutscher -- Important but little known (or forgotten) artifacts in protein biochemistry / R.R. Burgess |
| Summary |
The second edition of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology. Assembles chapters on both common and less-common relevant techniques. Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Description based on print version record |
| Subject |
Proteins -- Purification.
|
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Proteins -- isolation & purification.
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| Form |
Electronic book
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| Author |
Burgess, Richard R.
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|
Deutscher, Murray P.
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| ISBN |
0123745365 (electronic bk.) |
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9780123745361 (electronic bk.) |
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9780123749789 (paperback) |
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