Description |
1 online resource (streaming video file) (556 secs.) |
Series |
Biology |
Summary |
Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes |
Audience |
For undergraduate, graduate, and professional students |
Notes |
English |
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Description based on online resource; title from resource description page |
Subject |
Biology
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Form |
Streaming audio
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